Detection of two pathogenic marine ciliates Ancistrum haliotis and A. crassum (Ciliophora: Scuticociliatia) by fluorescence in situ hybridization

doi:10.1007/s13131-020-1683-0

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生物分类与系统演化实验室

	Detection of two pathogenic marine ciliates Ancistrum haliotis and A. crassum (Ciliophora: Scuticociliatia) by fluorescence in situ hybridization
	Zhan, Zifeng 2,3,4; Xu, Kuidong 1,2,3,4
刊名	ACTA OCEANOLOGICA SINICA
	2020-12-28
页码	5
关键词	oligonucleotide probes molecular methods scuticociliates SSU rRNA abalone mollusc
ISSN号	0253-505X
DOI	10.1007/s13131-020-1683-0
通讯作者	Xu, Kuidong(kxu@qdio.ac.cn)
英文摘要	The scuticociliatid ciliates Ancistrum haliotis and A. crassum are parasites that may cause high mortality in the cultured abalone Haliotis spp. and the bivalve Ruditapes philippinarum. Traditional identification with silver staining methods is hampered by their morphological similarities to closely related species and the complicated procedures of morphological analysis. We designed two SSU rRNA-targeted oligonucleotide probes labeled with a fluorochrome, and optimized the fluorescence in situ hybridization (FISH) protocols for identification of A. halioti and A. crassum, respectively. The assays resulted in a clear identification by strong fluorescence signals from the oligonucleotide probes. The method can be used for quick and accurate quantitative analysis of A. haliotis and A. crassum infections on host molluscs.
资助项目	National Natural Science Foundation of China[41876171] ; National Natural Science Foundation of China[41406171]
WOS研究方向	Oceanography
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000603188400002
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/169548]
专题	海洋研究所_海洋生物分类与系统演化实验室
通讯作者	Xu, Kuidong
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Lab Marine Organism Taxon & Phylogeny, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhan, Zifeng,Xu, Kuidong. Detection of two pathogenic marine ciliates Ancistrum haliotis and A. crassum (Ciliophora: Scuticociliatia) by fluorescence in situ hybridization[J]. ACTA OCEANOLOGICA SINICA,2020:5.
APA	Zhan, Zifeng,&Xu, Kuidong.(2020).Detection of two pathogenic marine ciliates Ancistrum haliotis and A. crassum (Ciliophora: Scuticociliatia) by fluorescence in situ hybridization.ACTA OCEANOLOGICA SINICA,5.
MLA	Zhan, Zifeng,et al."Detection of two pathogenic marine ciliates Ancistrum haliotis and A. crassum (Ciliophora: Scuticociliatia) by fluorescence in situ hybridization".ACTA OCEANOLOGICA SINICA (2020):5.