Molecular cloning and transcriptional regulation of two gamma-carbonic anhydrase genes in the green macroalga Ulva prolifera

doi:10.1007/s10709-020-00112-4

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Molecular cloning and transcriptional regulation of two gamma-carbonic anhydrase genes in the green macroalga Ulva prolifera
	Wang, Yu 4; Liu, Feng 1,2,4; Liu, Manman 4; Shi, Shitao 4; Bi, Yuping 3; Chen, Nansheng 1,2,4
刊名	GENETICA
	2021-01-15
页码	10
关键词	Ulva prolifera Carbonic anhydrase Environmental factor Green tide Transcriptional regulation
ISSN号	0016-6707
DOI	10.1007/s10709-020-00112-4
通讯作者	Liu, Feng(liufeng@qdio.ac.cn)
英文摘要	Ulva prolifera O.F. Muller (Ulvophyceae, Chlorophyta) is well known as a typical green-tide forming macroalga which has caused the world's largest macroalgal blooms in the Yellow Sea of China. In this study, two full-length gamma-carbonic anhydrase (gamma-CA) genes (Up gamma CA1 and Up gamma CA2) were cloned from U. prolifera. Up gamma CA1 has three conserved histidine residues, which act as an active site for binding a zinc metal ion. In Up gamma CA2, two of the three histidine residues were replaced by serine and arginine, respectively. The two gamma-CA genes are clustered together with other gamma-CAs in Chlorophyta with strong support value (100% bootstrap) in maximum likelihood (ML) phylogenetic tree. Quantitative real-time PCR (qRT-PCR) analysis showed that stressful environmental conditions markedly inhibited transcription levels of these two gamma-CA genes. Low pH value (pH 7.5) significantly increased transcription level of Up gamma CA2 not Up gamma CA1 at 12 h, whereas high pH value (pH 8.5) significantly inhibited the transcription of these two gamma-CA genes at 6 h. These findings enhanced our understanding on transcriptional regulation of gamma-CA genes in response to environmental factors in U. prolifera.
资助项目	National Natural Science Foundation of China[41876165] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA23050302/XDA23050403/XDB42000000] ; Key Research Program of Frontier Sciences, Chinese Academy of Sciences[QYZDB-SSW-DQC023] ; Major Scientific and Technological Innovation Project of Shandong Province[2019JZZY020706] ; Earmarked Fund for Modern Agro-industry Technology Research System in Shandong Province of China[SDAIT-26-09]
WOS研究方向	Genetics & Heredity
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000607995300001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/169971]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Liu, Feng
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266400, Shandong, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Marine Ecol & Environm Sci Lab, Qingdao 266237, Shandong, Peoples R China 3.Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yu,Liu, Feng,Liu, Manman,et al. Molecular cloning and transcriptional regulation of two gamma-carbonic anhydrase genes in the green macroalga Ulva prolifera[J]. GENETICA,2021:10.
APA	Wang, Yu,Liu, Feng,Liu, Manman,Shi, Shitao,Bi, Yuping,&Chen, Nansheng.(2021).Molecular cloning and transcriptional regulation of two gamma-carbonic anhydrase genes in the green macroalga Ulva prolifera.GENETICA,10.
MLA	Wang, Yu,et al."Molecular cloning and transcriptional regulation of two gamma-carbonic anhydrase genes in the green macroalga Ulva prolifera".GENETICA (2021):10.