Transcriptome analysis of phototransduction-related genes in tentacles of the sea cucumber Apostichopus japonicus

doi:10.1016/j.cbd.2020.100675

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Transcriptome analysis of phototransduction-related genes in tentacles of the sea cucumber Apostichopus japonicus
	Liu, Xiaolu 2,3,4,5; Lin, Chenggang 1,2,3,4; Sun, Lina 2,3,4; Liu, Shilin 2,3,4; Sun, Jingchun 2,3,4; Zhang, Libin 2,3,4; Yang, Hongsheng 1,2,3,4
刊名	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
	2020-06-01
卷号	34 页码:11
关键词	Apostichopus japonicus Tentacle Transcriptome Phototransduction Opsin
ISSN号	1744-117X
DOI	10.1016/j.cbd.2020.100675
通讯作者	Lin, Chenggang(Linchenggang@qdio.ac.cn)
英文摘要	The sea cucumber Apostichopus japonicus (Selenka)is a typical nocturnal echinoderm, which is believed to be almost completely dependent on light intensity for the regulation of endogenous rhythms. Under conditions of high light intensity, this species shows clear evidence of light avoidance behavior, seeking out shaded areas of reef in which to reside. In this study, we performed RNA-Seq analysis to examine the tentacle transcriptome of A. japonicus specimens that had been subjected to dark and light (5 min and 1 h) conditions. We specifically focused on detecting genes involved in opsin-based light perception, including opsins and members of phototransduction-related pathways. On the basis of comparisons with both vertebrate and invertebrate phototransduction pathways, we determined that components of two of the main metazoan phototransduction pathways were altered in response to illumination. Among the key phototransduction-related genes in tentacles, we identified retinol dehydrogenase, members of the dehydrogenase/reductase family, and myosin III, and also detected a pair of visual pigment-like receptors, peropsin and peropsin-like, the homologous genes of which are believed to have the same function but show opposite expression patterns in response to different light environments. In general, the up-regulation of key genes in sea cucumber exposed to illumination indicated that the tentacles can respond to differences in the light environment at the molecular level.
资助项目	National Science Foundation for Young Scientists of China[41606171] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0502] ; Science and Technology Service Network Program of Chinese Academy of Sciences[KFJ-STS-ZDTP-55]
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000537087700009
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/167629]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Lin, Chenggang
作者单位	1.Chinese Acad Sci, Innovat Seed Design, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 5.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
推荐引用方式 GB/T 7714	Liu, Xiaolu,Lin, Chenggang,Sun, Lina,et al. Transcriptome analysis of phototransduction-related genes in tentacles of the sea cucumber Apostichopus japonicus[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2020,34:11.
APA	Liu, Xiaolu.,Lin, Chenggang.,Sun, Lina.,Liu, Shilin.,Sun, Jingchun.,...&Yang, Hongsheng.(2020).Transcriptome analysis of phototransduction-related genes in tentacles of the sea cucumber Apostichopus japonicus.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,34,11.
MLA	Liu, Xiaolu,et al."Transcriptome analysis of phototransduction-related genes in tentacles of the sea cucumber Apostichopus japonicus".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 34(2020):11.