Optimal long-term seed storage conditions for the endangered seagrass Zostera japonica: implications for habitat conservation and restoration

doi:10.1186/s13007-019-0541-6

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Optimal long-term seed storage conditions for the endangered seagrass Zostera japonica: implications for habitat conservation and restoration
	Yue, Shidong 1,2,3; Zhang, Yu 1,2,3; Zhou, Yi 1,2,4; Xu, Shaochun 1,2,3; Xu, Shuai 1,2,3; Zhang, Xiaomei 1,2,4; Gu, Ruiting 1,2,3
刊名	PLANT METHODS
	2019-12-26
卷号	15 期号:1 页码:10
关键词	Seagrass Zostera japonica Seed Storage condition Temperature Salinity Desiccation sensitivity Conservation Restoration
DOI	10.1186/s13007-019-0541-6
英文摘要	Background: Seagrass meadows are recognized as critical and among the most vulnerable habitats on the planet. The alarming rates of decline in seagrass meadows have attracted the attention globally. There is an urgent need to develop techniques to restore and preserve these vital coastal ecosystems. So far little work has been done to develop effective long-term storage method for seagrass seeds. The seagrass Zostera japonica Asch. & Graebn is an endangered species in its native range. Here we utilized combinations of different storage times, salinities, and temperature to determine the most appropriate conditions for optimal seed storage. Results: Zostera japonica seeds were strongly desiccation sensitive, with a complete loss of viability after 24 h of desiccation. Therefore, long periods of exposure to air should be avoided to minimize seed mortality. In addition, Z. japonica seeds could not endure freezing conditions such as -5 degrees C. However, our results indicated that reduced storage temperature to 0 degrees C could effectively prolong the duration of dormancy of Z. japonica seeds. Seeds stored at 0 degrees C under a salinity of 40-60 psu showed relatively low seed loss, high seed vigor and fast seed germination, suggesting these to be optimal seed storage conditions. For example, after storage for 540 days (ca. 600 days since the seed collection from reproductive shoots in early October, 2016) at 0 degrees C under a salinity of 50 psu, seeds still had a considerable vigor, i.e. 57.8 +/- 16.8%. Conclusion: Our experiments demonstrated that seeds stored at 0 degrees C under a salinity of 40-60 psu could effectively prolong the duration of dormancy of Z. japonica seeds. The proposed technique is a simple and effective long-term storage method for Z. japonica seeds, which can then be used to aid future conservation, restoration and management of these sensitive and ecologically important habitat formers. The findings may also serve as useful reference for seed storage of other threatened seagrass species and facilitate their ex situ conservation and habitat restoration.
资助项目	National Key R&D Program of China[2019YFD0901302] ; National Science & Technology Basic Work Program[2015FY110600] ; key Research and Development Project of Shandong Province[2017GHY15111] ; NSFC-Shandong Joint Fund for Marine Science Research Centers[U1606404] ; National Natural Science Foundation of China[41606192/41176140] ; Key Research Project of Frontier Sciences of CAS[QYZDB-SSW-DQC041-1] ; Marine Engineering Standardization Construction Project[CCSOA-OEAS-2018-13] ; Taishan Scholars Program (Distinguished Taishan Scholars) ; Creative Team Project of the Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology[LMEES-CTSP-2018-1]
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
出版者	BMC
WOS记录号	WOS:000510638800002
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/165394]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Zhou, Yi
作者单位	1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Yue, Shidong,Zhang, Yu,Zhou, Yi,et al. Optimal long-term seed storage conditions for the endangered seagrass Zostera japonica: implications for habitat conservation and restoration[J]. PLANT METHODS,2019,15(1):10.
APA	Yue, Shidong.,Zhang, Yu.,Zhou, Yi.,Xu, Shaochun.,Xu, Shuai.,...&Gu, Ruiting.(2019).Optimal long-term seed storage conditions for the endangered seagrass Zostera japonica: implications for habitat conservation and restoration.PLANT METHODS,15(1),10.
MLA	Yue, Shidong,et al."Optimal long-term seed storage conditions for the endangered seagrass Zostera japonica: implications for habitat conservation and restoration".PLANT METHODS 15.1(2019):10.