A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of OysterCrassostrea gigas

doi:10.3389/fimmu.2020.01379

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of OysterCrassostrea gigas
	Liu, Conghui 2,3,4; Yang, Chuanyan 1,2,4; Wang, Mengqiang 3; Jiang, Shuai 3; Yi, Qilin 1,2,4; Wang, Weilin 1,2,3; Wang, Lingling 1,2,4; Song, Linsheng 2,4
刊名	FRONTIERS IN IMMUNOLOGY
	2020-07-22
卷号	11 页码:12
关键词	tetraspanin phagosomes recruitment receptor innate immune response Crassostrea gigas
ISSN号	1664-3224
DOI	10.3389/fimmu.2020.01379
通讯作者	Wang, Lingling(wanglingling@dlou.edu.cn) ; Song, Linsheng(lshsong@dlou.edu.cn)
英文摘要	Cluster of differentiation 63 (CD63), a four-transmembrane glycoprotein in the subfamily of tetraspanin, has been widely recognized as a gateway from the infection of foreign invaders to the immune defense of hosts. Its role in Pacific oysterCrassostrea gigasis, however, yet to be discovered. This work makes contributions by identifyingCgCD63H, a CD63 homolog with four transmembrane domains and one conservative CCG motif, and establishing its role as a receptor that participates in immune recognition and hemocyte phagocytosis. The presence ofCgCD63H messenger RNA (mRNA) in hepatopancreas, labial palps, gill, and hemocytes is confirmed. The expression level of mRNA in hemocytes is found significantly (p< 0.01) upregulated after the injection ofVibrio splendidus.CgCD63H protein, typically distributed over the plasma membrane of oyster hemocytes, is recruited to theYarrowia lipolytica-containing phagosomes after the stimulation ofY. lipolytica. The recombinantCgCD63H protein expresses binding capacity to glucan (GLU), peptidoglycan (PGN), and lipopolysaccharide (LPS) in the presence of lyophilized hemolymph. The phagocytic rate of hemocytes towardV. splendidusandY. lipolyticais significantly inhibited (p< 0.01) after incubation with anti-CgCD63H antibody. Our work further suggests thatCgCD63H functions as a receptor involved in the immune recognition and hemocyte phagocytosis against invading pathogen, which can be a marker candidate for the hemocyte typing inC. gigas.
资助项目	National Key RD Program[2018YFD0900606] ; National Science Foundation of China[U1706204] ; National Science Foundation of China[41961124009] ; Modern Agro-Industry Technology Research System[CARS-49] ; Fund for Outstanding Talents and Innovative Team of Agricultural Scientific Research from the Ministry of Agriculture and Rural Affairs in China ; AoShan Talents Cultivation Program - Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Liaoning Climbing Scholar ; Dalian High Level Talent Innovation Support Program[2015R020] ; Research Foundation for Talented Scholars in Dalian Ocean University ; [XLYC1902012]
WOS研究方向	Immunology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000559341100001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/168180]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wang, Lingling; Song, Linsheng
作者单位	1.Dalian Ocean Univ, Dalian Key Lab Aquat Anim Dis Prevent & Control, Dalian, Peoples R China 2.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 4.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Liu, Conghui,Yang, Chuanyan,Wang, Mengqiang,et al. A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of OysterCrassostrea gigas[J]. FRONTIERS IN IMMUNOLOGY,2020,11:12.
APA	Liu, Conghui.,Yang, Chuanyan.,Wang, Mengqiang.,Jiang, Shuai.,Yi, Qilin.,...&Song, Linsheng.(2020).A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of OysterCrassostrea gigas.FRONTIERS IN IMMUNOLOGY,11,12.
MLA	Liu, Conghui,et al."A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of OysterCrassostrea gigas".FRONTIERS IN IMMUNOLOGY 11(2020):12.