Optimization of Oyster (Crassostrea talienwhanensis) Protein Hydrolysates Using Response Surface Methodology

doi:10.3390/molecules25122844

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	Optimization of Oyster (Crassostrea talienwhanensis) Protein Hydrolysates Using Response Surface Methodology
	Wang, Xueqin 1,2; Yu, Huahua 1,2; Xing, Ronge 1,2; Liu, Song 1,2; Chen, Xiaolin 1,2; Li, Pengcheng 1,2
刊名	MOLECULES
	2020-06-01
卷号	25 期号:12 页码:14
关键词	oyster protein hydrolysate response surface methodology degree of hydrolysis hydroxyl-radical-scavenging activity branched-chain amino acids
DOI	10.3390/molecules25122844
通讯作者	Li, Pengcheng(pcli@qdio.ac.cn)
英文摘要	Oyster (Crassostrea talienwhanensis) protein was hydrolyzed by trypsin to produce peptides with different response values, and response surface methodology (RSM) was applied to optimize the hydrolysis conditions. The highest degree of hydrolysis (DH) of the oyster peptide (OP) was obtained at an enzyme concentration of 1593.2 U/g, a pH of 8.2, a hydrolysis temperature of 40.1 degrees C, a hydrolysis time of 6.0 h, and a water/material ratio of 8.2. The greatest hydroxyl-radical-scavenging activity of OP was obtained at an enzyme concentration of 1546.3 U/g, a pH of 9.0, a hydrolysis temperature of 50.2 degrees C, a hydrolysis time of 5.1 h, and a water/material ratio of 5.6. The largest branched-chain amino acid (BCAA) content of OP was obtained at an enzyme concentration of 1323.8 U/g, a pH of 8.3, a hydrolysis temperature of 41.7 degrees C, a hydrolysis time of 6.7 h, and a water/material ratio of 4.8. The three experimental values were significantly in agreement with the predicted values within the 95% confidence interval. Furthermore, ultrafiltration and chromatographic methods were used to purify the OP, and 13 peptides that were rich in Lys, Arg, His, and Thr were identified by LC-MS/MS. The results of this study offer different optimum hydrolysis conditions to produce target peptides from oyster protein by using RSM, and this study provide a theoretical basis for the high-value utilization of oyster protein.
资助项目	Key Research and Development Program of Shandong Province[2017YYSP018] ; STS Project of the Chinese Academy of Sciences[2017T3006]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	MDPI
WOS记录号	WOS:000550221500001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/167924]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Li, Pengcheng
作者单位	1.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xueqin,Yu, Huahua,Xing, Ronge,et al. Optimization of Oyster (Crassostrea talienwhanensis) Protein Hydrolysates Using Response Surface Methodology[J]. MOLECULES,2020,25(12):14.
APA	Wang, Xueqin,Yu, Huahua,Xing, Ronge,Liu, Song,Chen, Xiaolin,&Li, Pengcheng.(2020).Optimization of Oyster (Crassostrea talienwhanensis) Protein Hydrolysates Using Response Surface Methodology.MOLECULES,25(12),14.
MLA	Wang, Xueqin,et al."Optimization of Oyster (Crassostrea talienwhanensis) Protein Hydrolysates Using Response Surface Methodology".MOLECULES 25.12(2020):14.