Comparative transcriptome analysis reveals the potential influencing mechanism of dietary astaxanthin on growth and metabolism in Litopenaeus vannamei

doi:10.1016/j.aqrep.2019.100259

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Comparative transcriptome analysis reveals the potential influencing mechanism of dietary astaxanthin on growth and metabolism in Litopenaeus vannamei
	Wang, Yichao 2,3,5; Wang, Baojie 2,5; Liu, Mei 2,5; Jiang, Keyong 2,5; Wang, Mengqiang 2,4,5; Wang, Lei 1,2,5
刊名	AQUACULTURE REPORTS
	2020-03-01
卷号	16 页码:8
关键词	Astaxanthin Litopenaeus vannamei Transcriptome Growth performance Metabolism
ISSN号	2352-5134
DOI	10.1016/j.aqrep.2019.100259
通讯作者	Wang, Lei(leiwang@qdio.ac.cn)
英文摘要	The Pacific white shrimp (Litopenaeus vannamei), as one of the most valuable crustaceans, is widely distributed in China. Dietary astaxanthin has multiple functions in L. vannamei, such as promoting growth and antioxidant production. In the current study, shrimps were fed a diet which was supplemented with 0, 50, or 100 ppm astaxanthin for 4 weeks, and the specific growth rate (SGR) and weight gain were significantly greater in treatment groups compared to control group. We performed the transcriptome analysis of hepatopancreas of L. vannamei and detected 14,336, 14,780, and 14,400 genes, respectively. 598 and 285 genes were significant differentially expressed compared with the control group, under supplementation with 50 and 100 ppm astaxanthin, respectively, of which 434 and 104 were up-regulated and 164 and 181 were down-regulated. Through the KEGG pathway analysis, we found that the significant differentially expressed genes were mainly enriched in metabolic-related pathways, such as metabolic pathway, pyruvate metabolism, and glycolysis/gluconeogenesis pathway. Twelve genes were chosen to validate the results of RNA-Seq by qRT-PCR. This study provides valuable information about the effect of astaxanthin on L. vannamei. The genes and pathways, which are identified in the current study, can help to reveal the molecular mechanism responsible for the effect of astaxanthin on metabolism.
资助项目	National Natural Science Foundation of China-Joint Fund of Shandong People's Government[U1706209] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0502-2] ; Chinese Academy of Sciences STS Program Supporting Project[2017T3011]
WOS研究方向	Fisheries
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000517752000025
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/167065]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wang, Lei
作者单位	1.Chinese Acad Sci, CAS Ctr Ocean Megasci, Qingdao 266400, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Natl Lab Marine Sci & Technol, Res Platform Marine Mol Biotechnol, Qingdao 266237, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yichao,Wang, Baojie,Liu, Mei,et al. Comparative transcriptome analysis reveals the potential influencing mechanism of dietary astaxanthin on growth and metabolism in Litopenaeus vannamei[J]. AQUACULTURE REPORTS,2020,16:8.
APA	Wang, Yichao,Wang, Baojie,Liu, Mei,Jiang, Keyong,Wang, Mengqiang,&Wang, Lei.(2020).Comparative transcriptome analysis reveals the potential influencing mechanism of dietary astaxanthin on growth and metabolism in Litopenaeus vannamei.AQUACULTURE REPORTS,16,8.
MLA	Wang, Yichao,et al."Comparative transcriptome analysis reveals the potential influencing mechanism of dietary astaxanthin on growth and metabolism in Litopenaeus vannamei".AQUACULTURE REPORTS 16(2020):8.