Rapid detection of Enterocytozoon hepatopenaei in shrimp through an isothermal recombinase polymerase amplification assay

doi:10.1016/j.aquaculture.2020.734987

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Rapid detection of Enterocytozoon hepatopenaei in shrimp through an isothermal recombinase polymerase amplification assay
	Zhou, Shuhong 1,3; Wang, Mengqiang 1; Liu, Mei 1; Jiang, Keyong 1; Wang, Baojie 1; Wang, Lei 1,2,4
刊名	AQUACULTURE
	2020-05-15
卷号	521 页码:6
关键词	Penaeus vannamei Enterocytozoon hepatopenaei Recombinase polymerase amplification
ISSN号	0044-8486
DOI	10.1016/j.aquaculture.2020.734987
通讯作者	Wang, Baojie(wangbaojie@qdio.ac.cn) ; Wang, Lei(leiwang@qdio.ac.cn)
英文摘要	Enterocytozoon hepatopenaei (EHP) has caused mortality in Pacific white shrimp (Penaeus vannamei) and resulted in large economic losses in shrimp farming. The best way to avoid this parasitic disease is to find out about them early. In this study, we describe a novel, sensitive and specific rapid isothermal recombinase polymerase amplification assay (RPA) for detecting EHP. We evaluated the ability, including the specificity and sensitivity, of the EHP-RPA assay to detect EHP. By optimizing the reaction time and temperature, and screening primers based on the conserved small subunit ribosomal RNA gene, we determined the optimal reaction conditions. The RPA could be performed at 30 degrees C for 40 min without cross-reactivity with other pathogens. Sensitivity assay indicated that the limit of detection for EHP-RPA, PCR and real-time PCR was 8 x 10(2), 8 x 10(2) and 8 x 10(1) copies/mu L, respectively. Using 18 samples collected from shrimp farms, we demonstrated that RPA enables simple, convenient and rapid EHP detection, especially when laboratory equipment is lacking. This new RPA method is easy to implement and has great potential for use in both laboratory and field testing applications.
资助项目	National Key R&D Program of China[2019YFD0900401] ; National Natural Science Foundation of China-Joint Fund of Shandong People's Government[2017T3011]
WOS研究方向	Fisheries ; Marine & Freshwater Biology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000521282400061
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/166996]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wang, Baojie; Wang, Lei
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, CAS Ctr Ocean Megasci, Qingdao 266400, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Shuhong,Wang, Mengqiang,Liu, Mei,et al. Rapid detection of Enterocytozoon hepatopenaei in shrimp through an isothermal recombinase polymerase amplification assay[J]. AQUACULTURE,2020,521:6.
APA	Zhou, Shuhong,Wang, Mengqiang,Liu, Mei,Jiang, Keyong,Wang, Baojie,&Wang, Lei.(2020).Rapid detection of Enterocytozoon hepatopenaei in shrimp through an isothermal recombinase polymerase amplification assay.AQUACULTURE,521,6.
MLA	Zhou, Shuhong,et al."Rapid detection of Enterocytozoon hepatopenaei in shrimp through an isothermal recombinase polymerase amplification assay".AQUACULTURE 521(2020):6.