Identification of a laccase gene involved in shell periostracal tanning of the clam Meretrix petechialis

doi:10.3354/ab00709

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Identification of a laccase gene involved in shell periostracal tanning of the clam Meretrix petechialis
	Yue, Xin 1,2; Zhang, Shujing 1,3; Yu, Jiajia 1,3; Liu, Baozhong 1,2,3
刊名	AQUATIC BIOLOGY
	2019
卷号	28 页码:55-65
关键词	Meretrix petechialis Laccase Shell periostracum Tanning
ISSN号	1864-7790
DOI	10.3354/ab00709
通讯作者	Liu, Baozhong(bzliu@qdio.ac.cn)
英文摘要	Tanning is a complex extracellular process that is a mechanism for stabilizing proteinaceous extracellular structures. Phenoloxidases play important roles in cross-linking during tanning, and laccase (EC 1.10.3.2) is a member of the phenoloxidase enzyme class. In this study, we identified a laccase gene (MpLac) from the clam Meretrix petechialis and found that MpLac might be involved in shell periostracal tanning of clams. Using whole-mount in situ hybridization, we found that MpLac mRNA in the larval clam was mainly expressed in the mantle edge. In the adult clam, our quantitative real-time PCR results showed that the mantle was also a tissue with a high MpLac expression level; in addition, by combining the results of fluorescence in situ hybridization, H&E staining and transmission electron microscopy, we found that the inner epithelium of the outer fold of the mantle edge, which is involved in periostracum formation, was the exact region in which MpLac mRNA was expressed. Furthermore, knocking down the expression of MpLac by RNA interference (RNAi) bleached the new shell periostracum. All of our results suggest the involvement of MpLac in shell periostracal tanning of M. petechialis.
资助项目	China Agriculture Research System[CARS-49] ; Youth Foundation Project from Qingdao National Laboratory for Marine Science and Technology[YQ2018NO07] ; National Natural Science Foundation of China[NSFC 31772845]
WOS研究方向	Marine & Freshwater Biology
语种	英语
出版者	INTER-RESEARCH
WOS记录号	WOS:000518432200004
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/165547]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Liu, Baozhong
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1 Wenhai Rd, Qingdao 266000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yue, Xin,Zhang, Shujing,Yu, Jiajia,et al. Identification of a laccase gene involved in shell periostracal tanning of the clam Meretrix petechialis[J]. AQUATIC BIOLOGY,2019,28:55-65.
APA	Yue, Xin,Zhang, Shujing,Yu, Jiajia,&Liu, Baozhong.(2019).Identification of a laccase gene involved in shell periostracal tanning of the clam Meretrix petechialis.AQUATIC BIOLOGY,28,55-65.
MLA	Yue, Xin,et al."Identification of a laccase gene involved in shell periostracal tanning of the clam Meretrix petechialis".AQUATIC BIOLOGY 28(2019):55-65.