Transcriptome analysis reveals regulation of gene expression during photoacclimation to high irradiance levels in Dunaliella salina (Chlorophyceae)

doi:10.1111/pre.12379

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Transcriptome analysis reveals regulation of gene expression during photoacclimation to high irradiance levels in Dunaliella salina (Chlorophyceae)
	Li, Yuanxiang 1,2,3; Gu, Wenhui 1,2; Huang, Aiyou 1,2; Xie, Xiujun 1,2; Wu, Songcui 1,2; Wang, Guangce 1,2
刊名	PHYCOLOGICAL RESEARCH
	2019-10-01
卷号	67 期号:4 页码:291-302
关键词	carotenoid light treatment microalgae photosynthesis photosynthetic activity
ISSN号	1322-0829
DOI	10.1111/pre.12379
通讯作者	Wang, Guangce(gcwang@qdio.ac.cn)
英文摘要	The unicellular green alga Dunaliella salina is an attractive organism for studying photoacclimation responses. Changes in irradiance level during cell growth affect the organization and structure of the photosystem and the composition of pigments. In this study, D. salina was exposed to different irradiance levels, and cell growth, photosynthetic activity, pigments, and neutral lipids were analyzed. Photosystem II activity and chlorophyll content were reduced, carotenoids content and neutral lipids increased as light intensity increased, suggesting that photosynthetic apparatus was reduced and photoprotection mechanisms were activated. The RNA of D. salina was sequenced to investigate the transcriptomic response of the organism after transitioning from normal light conditions to higher light intensity. Genes encoding for enzymes involved in photosynthesis were downregulated, whereas genes involved in the metabolism of carotenoid and triacylglycerol were upregulated. Genes encoding for photoprotective enzymes related to reactive oxygen species scavenging and to the xanthophyll cycle were also upregulated at higher irradiance levels. The present transcriptomic study would assist in the comprehensive understanding of photoacclimation mechanisms of D. salina.
资助项目	National Natural Science Foundation of China[41506188] ; China Nantong Municipal Applied Basic Research Program[MS12017025-2] ; Tianjin Demonstration Project for Innovative Development of Marine Economy[BHSF2017-21]
WOS研究方向	Marine & Freshwater Biology
语种	英语
出版者	WILEY
WOS记录号	WOS:000491488900005
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/164868]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wang, Guangce
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Li, Yuanxiang,Gu, Wenhui,Huang, Aiyou,et al. Transcriptome analysis reveals regulation of gene expression during photoacclimation to high irradiance levels in Dunaliella salina (Chlorophyceae)[J]. PHYCOLOGICAL RESEARCH,2019,67(4):291-302.
APA	Li, Yuanxiang,Gu, Wenhui,Huang, Aiyou,Xie, Xiujun,Wu, Songcui,&Wang, Guangce.(2019).Transcriptome analysis reveals regulation of gene expression during photoacclimation to high irradiance levels in Dunaliella salina (Chlorophyceae).PHYCOLOGICAL RESEARCH,67(4),291-302.
MLA	Li, Yuanxiang,et al."Transcriptome analysis reveals regulation of gene expression during photoacclimation to high irradiance levels in Dunaliella salina (Chlorophyceae)".PHYCOLOGICAL RESEARCH 67.4(2019):291-302.