A Comparative Analysis of Edwardsiella tarda-Induced Transcriptome Profiles in RAW264.7 Cells Reveals New Insights into the Strategy of Bacterial Immune Evasion

doi:10.3390/ijms20225724

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	A Comparative Analysis of Edwardsiella tarda-Induced Transcriptome Profiles in RAW264.7 Cells Reveals New Insights into the Strategy of Bacterial Immune Evasion
	Li, Huili 1,2,3; Sun, Boguang 1,2; Ning, Xianhui 1,2; Jiang, Shuai 1,2; Sun, Li 1,2
刊名	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2019-11-01
卷号	20 期号:22 页码:20
关键词	Edwardsiella tarda macrophage infection transcriptome immune evasion
DOI	10.3390/ijms20225724
通讯作者	Sun, Li(lsun@qdio.ac.cn)
英文摘要	Edwardsiella tarda is a Gram-negative bacterial pathogen with a broad host range, including fish, reptiles, and mammals. One prominent virulence feature of E. tarda is its ability to survive and replicate in host phagocytes, but the relevant molecular mechanism is largely unknown. In this study, we examined the transcriptome profiles of RAW264.7 cells, a murine macrophage cell line, infected with live E. tarda or stimulated with dead E. tarda for 4 h and 8 h. Eighteen libraries were constructed, and an average of 69 million clean reads per library were obtained, with similar to 81.63% of the reads being successfully mapped to the reference genome. In total, 208 and 232 differentially expressed genes (DEGs) were identified between live and dead E. tarda-treated cells at 4 h and 8 h post-infection, respectively. The DEGs were markedly enriched in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways associated with immunity. Live E. tarda differed strikingly from dead E. tarda in the regulation of immune related genes. Compared with dead E. tarda-treated cells, live E. tarda-treated cells exhibited marked and significant suppression in the induction of a large amount of immune genes, including RIG-I-like receptors, cytokines, and interferon-related genes. Furthermore, some of the immune genes highly regulated by live E. tarda formed complicated interaction networks with each other. Together, the results of this study revealed a transcriptome profile specifically induced by the active virulence elements of live E. tarda during the infection process, thus adding new insights into the intracellular infection mechanism of E. tarda. This study also provided a valuable set of target genes for further study of the immune evasion strategy of E. tarda.
资助项目	National Key R&D Program of China[2018YFD0900500] ; Shandong Major Science and Technology Innovation Project[2018SDKJ0302-2] ; AoShan Talents Program ; Qingdao National Laboratory for Marine Science and Technology[2015ASTP] ; Taishan Scholar Program of Shandong Province
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	MDPI
WOS记录号	WOS:000502786800206
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/164226]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Sun, Li
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1Wenhai Rd, Qingdao 266237, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Huili,Sun, Boguang,Ning, Xianhui,et al. A Comparative Analysis of Edwardsiella tarda-Induced Transcriptome Profiles in RAW264.7 Cells Reveals New Insights into the Strategy of Bacterial Immune Evasion[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2019,20(22):20.
APA	Li, Huili,Sun, Boguang,Ning, Xianhui,Jiang, Shuai,&Sun, Li.(2019).A Comparative Analysis of Edwardsiella tarda-Induced Transcriptome Profiles in RAW264.7 Cells Reveals New Insights into the Strategy of Bacterial Immune Evasion.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(22),20.
MLA	Li, Huili,et al."A Comparative Analysis of Edwardsiella tarda-Induced Transcriptome Profiles in RAW264.7 Cells Reveals New Insights into the Strategy of Bacterial Immune Evasion".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.22(2019):20.