Transcriptome sequencing of Saccharina japonica sporophytes during whole developmental periods reveals regulatory networks underlying alginate and mannitol biosynthesis

doi:10.1186/s12864-019-6366-x

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Transcriptome sequencing of Saccharina japonica sporophytes during whole developmental periods reveals regulatory networks underlying alginate and mannitol biosynthesis
	Shao, Zhanru 1,2; Zhang, Pengyan 1,2,3; Lu, Chang 1,2,4; Li, Shaoxuan 5; Chen, Zhihang 1,2,4; Wang, Xiuliang 1,2; Duan, Delin 1,2,6
刊名	BMC GENOMICS
	2019-12-12
卷号	20 期号:1 页码:15
关键词	Alginate Mannitol Transcriptome Regulatory networks Growth Development Saccharina japonica
ISSN号	1471-2164
DOI	10.1186/s12864-019-6366-x
英文摘要	Background Alginate is an important cell wall component and mannitol is a soluble storage carbon substance in the brown seaweed Saccharina japonica. Their contents vary with kelp developmental periods and harvesting time. Alginate and mannitol regulatory networks and molecular mechanisms are largely unknown. Results With WGCNA and trend analysis of 20,940 known genes and 4264 new genes produced from transcriptome sequencing of 30 kelp samples from different stages and tissues, we deduced that ribosomal proteins, light harvesting complex proteins and "imm upregulated 3" gene family are closely associated with the meristematic growth and kelp maturity. Moreover, 134 and 6 genes directly involved in the alginate and mannitol metabolism were identified, respectively. Mannose-6-phosphate isomerase (MPI2), phosphomannomutase (PMM1), GDP-mannose 6-dehydrogenase (GMD3) and mannuronate C5-epimerase (MC5E70 and MC5E122) are closely related with the high content of alginate in the distal blade. Mannitol accumulation in the basal blade might be ascribed to high expression of mannitol-1-phosphate dehydrogenase (M1PDH1) and mannitol-1-phosphatase (M1Pase) (in biosynthesis direction) and low expression of mannitol-2-dehydrogenase (M2DH) and Fructokinase (FK) (in degradation direction). Oxidative phosphorylation and photosynthesis provide ATP and NADH for mannitol metabolism whereas glycosylated cycle and tricarboxylic acid (TCA) cycle produce GTP for alginate biosynthesis. RNA/protein synthesis and transportation might affect alginate complex polymerization and secretion processes. Cryptochrome (CRY-DASH), xanthophyll cycle, photosynthesis and carbon fixation influence the production of intermediate metabolite of fructose-6-phosphate, contributing to high content of mannitol in the basal blade. Conclusions The network of co-responsive DNA synthesis, repair and proteolysis are presumed to be involved in alginate polymerization and secretion, while upstream light-responsive reactions are important for mannitol accumulation in meristem of kelp. Our transcriptome analysis provides new insights into the transcriptional regulatory networks underlying the biosynthesis of alginate and mannitol during S. japonica developments.
资助项目	Shandong Key Research and Development Program[2018GHY115023] ; Qingdao National Laboratory for Marine Science and Technology Project[2018ASKJ03] ; National Natural Science Foundation of China[41806175] ; Open Foundation of the State Key Laboratory of Bioactive Seaweed Substances[SKL-BASS1702]
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
语种	英语
出版者	BMC
WOS记录号	WOS:000502786600002
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/164145]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Duan, Delin
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100093, Peoples R China 5.Qingdao Acad Agr Sci, Qingdao 266100, Shandong, Peoples R China 6.Qingdao Brightmoon Seaweed Grp Co Ltd, State Key Lab Bioact Seaweed Subst, Qingdao 266400, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Shao, Zhanru,Zhang, Pengyan,Lu, Chang,et al. Transcriptome sequencing of Saccharina japonica sporophytes during whole developmental periods reveals regulatory networks underlying alginate and mannitol biosynthesis[J]. BMC GENOMICS,2019,20(1):15.
APA	Shao, Zhanru.,Zhang, Pengyan.,Lu, Chang.,Li, Shaoxuan.,Chen, Zhihang.,...&Duan, Delin.(2019).Transcriptome sequencing of Saccharina japonica sporophytes during whole developmental periods reveals regulatory networks underlying alginate and mannitol biosynthesis.BMC GENOMICS,20(1),15.
MLA	Shao, Zhanru,et al."Transcriptome sequencing of Saccharina japonica sporophytes during whole developmental periods reveals regulatory networks underlying alginate and mannitol biosynthesis".BMC GENOMICS 20.1(2019):15.