Dietary beta-glucan modulate haematological parameters, cytokines and gene expression in TLR and ERK pathways of rainbow trout (Oncorhynchus mykiss) during infection by Aeromonas salmonicida

doi:10.1111/are.14436

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Dietary beta-glucan modulate haematological parameters, cytokines and gene expression in TLR and ERK pathways of rainbow trout (Oncorhynchus mykiss) during infection by Aeromonas salmonicida
	Ji, Liqin 2,3,4,5; Fu, Songzhe 1; Sun, Guoxiang 3; Li, Xian 2,3,4; Liu, Ying 1,4
刊名	AQUACULTURE RESEARCH
	2019-12-13
页码	12
关键词	Aeromonas salmonicida cytokines rainbow trout TLR and ERK signalling pathways beta-glucans
ISSN号	1355-557X
DOI	10.1111/are.14436
英文摘要	To explore the role of beta-glucan (0, 0.05%, 0.1% and 0.2%) in resisting bacteria, rainbow trout (Oncorhynchus mykiss) was challenged with Aeromonas salmonicida after 42-day beta-glucan administration, and then sampled at 0, 2, 4 and 6 days post infection (dpi). The data showed that 0.2% beta-glucan reduced the accumulated mortality rates compared with the ICG (infected control group) (p ). The white blood cells, red blood cells and haemoglobin were higher in the 0.2% beta-glucan group (BG) than the ICG (p ). 0.1% and 0.2% beta-glucan elevated serum total antioxidative capability and glutathione activity but alleviated the increase of serum alkaline phosphatase activity and glucose concentration (p < .05) during infection. Serum TNF-alpha, IL-1 beta, IL-8 and IgM in three BGs elevated remarkably on 6 dpi compared with the ICG (p ). Expression of tnf, il1b and cxcl8 of the head kidney in the 0.1% and 0.2% BGs was higher than the ICG on 4 dpi while ighm expression in the 0.2% BG was higher than in the ICG on 2 and 6 dpi (p ). 0.1% and 0.2% beta-glucan increased the expression of tlr5m, tlr5s, tmek and myd88 in the spleen after infection (p < .05). Similarly, 0.2% beta-glucan up-regulated the expression of tmek, myd88, oncmyk-dab and c3 in head kidney (p < .05). Overall, 0.2% dietary beta-glucan effectively decreased accumulated mortality rate by modulating the biochemistry process, cytokines, and activating TLR and ERK signalling pathways during A. salmonicida infection.
资助项目	National Key R&D Program of China[2017YFD0701700] ; National Key Technologies RD Program[2017YFB0404000] ; Key Technologies R&D Program of Shandong Province[2018GHY115031] ; Key R&D Program of Guangdong Province[2019B020215001] ; National Natural Science Founds[31672673]
WOS研究方向	Fisheries
语种	英语
出版者	WILEY
WOS记录号	WOS:000502358400001
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/163750]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Liu, Ying
作者单位	1.Dalian Ocean Univ, Sch Marine Sci & Environm Engn, 52 Heroitte St, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Shandong, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 5.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Ji, Liqin,Fu, Songzhe,Sun, Guoxiang,et al. Dietary beta-glucan modulate haematological parameters, cytokines and gene expression in TLR and ERK pathways of rainbow trout (Oncorhynchus mykiss) during infection by Aeromonas salmonicida[J]. AQUACULTURE RESEARCH,2019:12.
APA	Ji, Liqin,Fu, Songzhe,Sun, Guoxiang,Li, Xian,&Liu, Ying.(2019).Dietary beta-glucan modulate haematological parameters, cytokines and gene expression in TLR and ERK pathways of rainbow trout (Oncorhynchus mykiss) during infection by Aeromonas salmonicida.AQUACULTURE RESEARCH,12.
MLA	Ji, Liqin,et al."Dietary beta-glucan modulate haematological parameters, cytokines and gene expression in TLR and ERK pathways of rainbow trout (Oncorhynchus mykiss) during infection by Aeromonas salmonicida".AQUACULTURE RESEARCH (2019):12.