High-Quality Genome Assembly of Eriocheir japonica sinensis Reveals Its Unique Genome Evolution

doi:10.3389/fgene.2019.01340

CORC > 海洋研究所 > 中国科学院海洋研究所

	High-Quality Genome Assembly of Eriocheir japonica sinensis Reveals Its Unique Genome Evolution
	Tang, Boping 9; Wang, Zhongkai 8; Liu, Qiuning 9; Zhang, Huabin 9; Jiang, Senhao 9; Li, Xinzheng 7; Wang, Zhengfei 9; Sun, Yue 9; Sha, Zhongli 7; Jiang, Hui 5,6
刊名	FRONTIERS IN GENETICS
	2020-01-17
卷号	10 页码:9
关键词	Eriocheir japonica sinensis evolution nanopore crab genome assembly and annotation
DOI	10.3389/fgene.2019.01340
通讯作者	Wang, Qun(qwang@bio.ecnu.edu.cn) ; Qiu, Gaofeng(gfqiu@shou.edu.cn) ; Zhang, Daizhen(daizhen79wenxin@163.com) ; Li, Yongxin(yxli28science@sina.com)
英文摘要	As an important freshwater aquaculture species in China, the Chinese mitten crab (Eriocheir japonica sinensis) has high economic and nutritional value. However, limited genomic information is currently available for studying its basic development and genetic diversity. Here, we performed whole-genome sequencing on Oxford Nanopore Technologies Limited's platform using promethION. The assembled size of E. j.sinensis genome was approximately 1.27 Gb, which is close to the estimated size (1.19 Gb). Furthermore, based on assessment using Benchmarking Universal Single-Copy Orthologs (BUSCO) (Simao et al., 2015), 94.00% of the expected eukaryotic genes were completely present in the genome assembly. In addition, repetitive sequences accounted for similar to 61.42% of the assembled genome, and 22,619 protein-coding genes were annotated. Comparative genomics analysis demonstrated that the Chinese mitten crab diverged from Penaeus vannamei similar to 373.6 million years ago, with a faster evolution rate than shrimp. We anticipate that the annotated high-quality genome of E. j. sinensis will promote research on its basic development and evolution and make substantial contributions to comparative genomic analyses of crustaceans.
资助项目	National Natural Science Foundation of China[31672267] ; National Natural Science Foundation of China[31640074] ; Jiangsu Agriculture Science and Technology Innovation Fund[CX(18) 3027] ; Natural Science Foundation of Jiangsu Province[BK20171276] ; Natural Science Foundation of Jiangsu Province[BK20160444] ; China Postdoctoral Science Foundation[2018M642105] ; National Key R&D Programof China[2018YFD0900201] ; Qing Lan Project of Daizhen Zhang
WOS研究方向	Genetics & Heredity
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000510907600001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/165334]
专题	中国科学院海洋研究所
通讯作者	Wang, Qun; Qiu, Gaofeng; Zhang, Daizhen; Li, Yongxin
作者单位	1.East China Normal Univ, Sch Life Sci, Dept Biol, Shanghai, Peoples R China 2.Nanjing Normal Univ, Coll Life Sci, Nanjing, Peoples R China 3.China Hong Kong Ecol Consultant Co, Hong Kong, Peoples R China 4.Shanghai Ocean Univ, Key Lab Freshwater Aquat Genet Resources, Minist Agr & Rural Affairs, Shanghai, Peoples R China 5.Zhejiang Ocean Univ, Natl Engn Res Ctr Facilitated Marine Aquaculture, Zhoushan, Peoples R China 6.Zhejiang Ocean Univ, Natl Engn Lab Marine Germplasm Resources Explorat, Zhoushan, Peoples R China 7.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 8.Northwestern Polytech Univ, Ctr Ecol & Environm Sci, Xian, Peoples R China 9.Yancheng Teachers Univ, Jiangsu Synthet Innovat Ctr Coastal Bioagr, Jiangsu Prov Key Lab Coastal Wetland Bioresources, Jiangsu Key Lab Bioresources Saline Soils, Yancheng, Peoples R China
推荐引用方式 GB/T 7714	Tang, Boping,Wang, Zhongkai,Liu, Qiuning,et al. High-Quality Genome Assembly of Eriocheir japonica sinensis Reveals Its Unique Genome Evolution[J]. FRONTIERS IN GENETICS,2020,10:9.
APA	Tang, Boping.,Wang, Zhongkai.,Liu, Qiuning.,Zhang, Huabin.,Jiang, Senhao.,...&Li, Yongxin.(2020).High-Quality Genome Assembly of Eriocheir japonica sinensis Reveals Its Unique Genome Evolution.FRONTIERS IN GENETICS,10,9.
MLA	Tang, Boping,et al."High-Quality Genome Assembly of Eriocheir japonica sinensis Reveals Its Unique Genome Evolution".FRONTIERS IN GENETICS 10(2020):9.