Effects of inorganic carbon and light on acetate assimilation by Nannochloropsis oceanica (Eustigmatophyceae) in mixotrophic cultivation

doi:10.1080/09670262.2019.1660808

CORC > 海洋研究所 > 中国科学院海洋研究所

	Effects of inorganic carbon and light on acetate assimilation by Nannochloropsis oceanica (Eustigmatophyceae) in mixotrophic cultivation
	Li, Yuanxiang 1,2,3; Huang, Aiyou 2,3; Gu, Wenhui 2,3; Wu, Songcui 2,3; Xie, Xiujun 2,3; Wang, Guangce 2,3
刊名	EUROPEAN JOURNAL OF PHYCOLOGY
	2020-01-02
卷号	55 期号:1 页码:64-75
关键词	C-13 metabolic analysis light mixotrophic Nannochloropsis sodium acetate sodium bicarbonate
ISSN号	0967-0262
DOI	10.1080/09670262.2019.1660808
通讯作者	Wang, Guangce(gcwang@qdio.ac.cn)
英文摘要	Microalgae are capable of accumulating biomass and high value-added products in mixotrophic culture. Carbon sources and light intensity have significant impacts on mixotrophic algal growth and metabolites accumulation. To investigate the underlying mechanism of interaction between metabolism of inorganic carbon and organic carbon, growth of Nannochloropsis oceanica with two different carbon sources (organic sodium acetate and inorganic sodium bicarbonate) and under three different light conditions (low, medium and high) was examined. A combination of sodium acetate and sodium bicarbonate significantly enhanced algal growth with higher acetate assimilation, when compared with single carbon sources. With increasing light intensity from low to high, the algal growth rates decreased in both autotrophic and mixotrophic cultures. Proteome and C-13 metabolic flux results showed that acetate utilization via the glyoxylate (GOX) cycle was enhanced, and both the tricarboxylic acid (TCA) cycle and putative C-4 pathway were stimulated in the presence of sodium acetate and sodium bicarbonate. These data suggest that sodium bicarbonate could facilitate acetate assimilation and enhance the putative C-4 pathway, GOX cycle and TCA cycle in N. oceanica under mixotrophic culture conditions.
资助项目	National Natural Science Foundation of China[41876158] ; National Natural Science Foundation of China[41806171] ; National Key R&D Program of China[2018YFD0901500] ; Haikou Marine Economic Demonstration City Industry Chain Collaborative Innovation Project[HHCL201811] ; Tianjin Marine Economic Demonstration[BHSF2017-21]
WOS研究方向	Plant Sciences ; Marine & Freshwater Biology
语种	英语
出版者	TAYLOR & FRANCIS LTD
WOS记录号	WOS:000520076900007
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/165076]
专题	中国科学院海洋研究所
通讯作者	Wang, Guangce
作者单位	1.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Li, Yuanxiang,Huang, Aiyou,Gu, Wenhui,et al. Effects of inorganic carbon and light on acetate assimilation by Nannochloropsis oceanica (Eustigmatophyceae) in mixotrophic cultivation[J]. EUROPEAN JOURNAL OF PHYCOLOGY,2020,55(1):64-75.
APA	Li, Yuanxiang,Huang, Aiyou,Gu, Wenhui,Wu, Songcui,Xie, Xiujun,&Wang, Guangce.(2020).Effects of inorganic carbon and light on acetate assimilation by Nannochloropsis oceanica (Eustigmatophyceae) in mixotrophic cultivation.EUROPEAN JOURNAL OF PHYCOLOGY,55(1),64-75.
MLA	Li, Yuanxiang,et al."Effects of inorganic carbon and light on acetate assimilation by Nannochloropsis oceanica (Eustigmatophyceae) in mixotrophic cultivation".EUROPEAN JOURNAL OF PHYCOLOGY 55.1(2020):64-75.