Silver nanoparticles exert concentration-dependent influences on biofilm development and architecture

doi:10.1111/cpr.12616

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Silver nanoparticles exert concentration-dependent influences on biofilm development and architecture
	Guo, JY ; Qin, SM ; Wei, Y ; Liu, SM ; Peng, HZ ; Li, QN ; Luo, LQ ; Lv, M
刊名	CELL PROLIFERATION
	2019
卷号	52 期号:4 页码:-
关键词	ANTIBIOTIC TOLERANCE IN-VITRO RESISTANCE NANOSTRUCTURES NANOMATERIALS EVOLUTION BACTERIA EXTRACT CURLI OXIDE
ISSN号	0960-7722
DOI	10.1111/cpr.12616
文献子类	期刊论文
英文摘要	Objectives To investigate the impact of silver nanoparticles (AgNPs) on the biofilm growth and architecture. Materials and methods Silver nitrate was reduced by d-maltose to prepare AgNPs in the presence of ammonia and sodium hydroxide. The physicochemical properties of AgNPs were characterized by transmission electron microscopy, ultraviolet-visible spectroscopy and inductively coupled plasma mass spectrometry. The development of biofilm with and without AgNPs was explored by crystal violet stain. The structures of mature biofilm were visually studied by confocal laser scanning microscopy and scanning electron microscopy. Bacterial cell, polysaccharide and protein within biofilm were assessed quantitatively by colony-counting method, phenol-sulphuric acid method and Bradford assay, respectively. Results The spherical AgNPs (about 30 nm) were successfully synthesized. The effect of AgNPs on Pseudomonas aeruginosa biofilm development was concentration-dependent. Biofilm was more resistant to AgNPs than planktonic cells. Low doses of AgNPs exposure remarkably delayed the growth cycle of biofilm, whereas high concentration (18 mu g/mL) of AgNPs fully prevented biofilm development. The analysis of biofilm architecture at the mature stage demonstrated that AgNPs exposure at all concentration led to significant decrease of cell viability within treated biofilms. However, sublethal doses of AgNPs increased the production of both polysaccharide and protein compared to control, which significantly changed the biofilm structure. Conclusions AgNPs exert concentration-dependent influences on biofilm development and structure, which provides new insight into the role of concentration played in the interaction between antibacterial nanoparticles and biofilm, especially, an ignored sublethal concentration associated with potential unintended consequences.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/32202]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai, Peoples R China 2.Shanghai Univ, Coll Sci, Shanghai, Peoples R China; 3.Chinese Acad Sci, Shanghai Synchrotron Radiat Facil, CAS Key Lab Interfacial Phys & Technol, Div Phys Biol,Shanghai Inst Appl Phys, Shanghai, Peoples R China; 4.Chinese Acad Sci, Shanghai Synchrotron Radiat Facil, CAS Key Lab Interfacial Phys & Technol, Bioimaging Ctr,Shanghai Inst Appl Phys, Shanghai, Peoples R China; 5.Univ Chinese Acad Sci, Beijing, Peoples R China; 6.Fudan Univ, Sch Publ Hlth, Key Lab Hlth Technol Assessment, Natl Hlth Commiss, Shanghai, Peoples R China; 7.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai, Peoples R China;
推荐引用方式 GB/T 7714	Guo, JY,Qin, SM,Wei, Y,et al. Silver nanoparticles exert concentration-dependent influences on biofilm development and architecture[J]. CELL PROLIFERATION,2019,52(4):-.
APA	Guo, JY.,Qin, SM.,Wei, Y.,Liu, SM.,Peng, HZ.,...&Lv, M.(2019).Silver nanoparticles exert concentration-dependent influences on biofilm development and architecture.CELL PROLIFERATION,52(4),-.
MLA	Guo, JY,et al."Silver nanoparticles exert concentration-dependent influences on biofilm development and architecture".CELL PROLIFERATION 52.4(2019):-.