Evaluating the biochemical changes of LPS-stimulated endothelial cells by synchrotron FTIR microspectroscopy at a single-cell level

doi:10.1007/s00216-019-02083-8

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Evaluating the biochemical changes of LPS-stimulated endothelial cells by synchrotron FTIR microspectroscopy at a single-cell level
	Wang, Y ; Wang, YD ; Huang, LN ; Liu, XY ; Hu, J ; L?, JH
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2019
卷号	411 期号:27 页码:7157-7164
关键词	GLYCOCALYX DAMAGE SPECTROSCOPY
ISSN号	1618-2642
DOI	10.1007/s00216-019-02083-8
文献子类	期刊论文
英文摘要	Endothelial damage is a major manifestation in many forms of heart and lung injuries induced by lipopolysaccharide (LPS), but the biochemical responses and activation mechanisms of endothelial cells have not been fully explicit. In this study, the biochemical changes to endothelial cells exposed to LPS were investigated by synchrotron FTIR microspectroscopy at a single-cell level. We found that the whole infrared spectrum of endothelial cells shifted after LPS treatment, indicating chemical component changes within cells. Principal component analysis (PCA) and t tests on subspectra (fatty acid region, protein region, and nucleic acid-sugar region, respectively) further showed that sugar components as well as fatty acids changed dramatically while proteins had no significant variation following LPS exposure. These results suggested that the glycocalyx layer structure on endothelial cell membrane may be mainly influenced by LPS and also proved that synchrotron FTIR microspectroscopy was a useful technique to evaluate the biochemical changes of endothelial damage at the single-cell level. Graphical abstract
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/32047]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Binzhou Med Univ, Coll Pharm, Yantai 264003, Shandong, Peoples R China; 2.Chinese Acad Sci, Shanghai Adv Res Inst, Zhangjiang Lab, Shanghai 201210, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai 201800, Peoples R China; 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Y,Wang, YD,Huang, LN,et al. Evaluating the biochemical changes of LPS-stimulated endothelial cells by synchrotron FTIR microspectroscopy at a single-cell level[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2019,411(27):7157-7164.
APA	Wang, Y,Wang, YD,Huang, LN,Liu, XY,Hu, J,&L?, JH.(2019).Evaluating the biochemical changes of LPS-stimulated endothelial cells by synchrotron FTIR microspectroscopy at a single-cell level.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,411(27),7157-7164.
MLA	Wang, Y,et al."Evaluating the biochemical changes of LPS-stimulated endothelial cells by synchrotron FTIR microspectroscopy at a single-cell level".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 411.27(2019):7157-7164.