B-cell epitopes: Discontinuity and conformational analysis

doi:10.1016/j.molimm.2019.09.014

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	B-cell epitopes: Discontinuity and conformational analysis
	Ferdous, S ; Kelm, S ; Baker, TS ; Shi, JY ; Martin, ACR
刊名	MOLECULAR IMMUNOLOGY
	2019
卷号	114 页码:643-650
关键词	ANTIGENIC DETERMINANTS FINAL STAGES PROTEIN PREDICTION VACCINE SUBUNIT REGIONS RECOGNITION PEPTIDES SCAFFOLD
ISSN号	0161-5890
DOI	10.1016/j.molimm.2019.09.014
文献子类	期刊论文
英文摘要	Peptide vaccines have many potential advantages over conventional ones including low cost, lack of need for cold-chain storage, safety and specificity. However, it is well known that approximately 90% of B-cell epitopes (BCEs) are discontinuous in nature making it difficult to mimic them for creating vaccines. In this study, the degree of discontinuity in B-cell epitopes and their conformational nature is examined. The discontinuity of B-cell epitopes is analyzed by defining 'regions' (consisting of at least three antibody-contacting residues each separated by <= 3 residues) and small fragments (antibody-contacting residues that do not satisfy the requirements for a region). Secondly, an algorithm has been developed that classifies each region's shape as straight, curved or folded on the basis that straight and folded regions are more likely to retain their native conformation as isolated peptides. We have investigated the structures of 488 B-cell epitopes from which 1282 regions and 1018 fragments have been identified. 90% of epitopes have five or fewer regions and five or fewer fragments with 14% containing only one region and 4% being truly linear (i.e. having one region and no fragments). Of the 1282 regions, 508 are straight in shape, 626 are curved and 148 are folded.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/31973]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Univ Manchester, Canc Res UK Manchester Inst, Alderley Pk, Macclesfield SK10 4TG, Cheshire, England 2.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China; 3.UCB Pharma, 216 Bath Rd, Slough SL1 4EN, Berks, England; 4.UCL, Inst Struct & Mol Biol, Div Biosci, Darwin Bldg,Gower St, London WC1E 6BT, England;
推荐引用方式 GB/T 7714	Ferdous, S,Kelm, S,Baker, TS,et al. B-cell epitopes: Discontinuity and conformational analysis[J]. MOLECULAR IMMUNOLOGY,2019,114:643-650.
APA	Ferdous, S,Kelm, S,Baker, TS,Shi, JY,&Martin, ACR.(2019).B-cell epitopes: Discontinuity and conformational analysis.MOLECULAR IMMUNOLOGY,114,643-650.
MLA	Ferdous, S,et al."B-cell epitopes: Discontinuity and conformational analysis".MOLECULAR IMMUNOLOGY 114(2019):643-650.