A guide to sample delivery systems for serial crystallography

doi:10.1111/febs.15099

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	A guide to sample delivery systems for serial crystallography
	Zhao, FZ ; Zhang, B ; Yan, EK ; Sun, B ; Wang, ZJ ; He, JH ; Yin, DC
刊名	FEBS JOURNAL
	2019
卷号	286 期号:22 页码:4402-4417
关键词	X-RAY-DIFFRACTION ROOM-TEMPERATURE FEMTOSECOND CRYSTALLOGRAPHY PROTEIN CRYSTALLIZATION STRUCTURAL BIOLOGY MILLISECOND CRYSTALLOGRAPHY CRYSTAL-STRUCTURE RADIATION-DAMAGE DATA-COLLECTION MICROCRYSTALS
ISSN号	1742-464X
DOI	10.1111/febs.15099
文献子类	期刊论文
英文摘要	Crystallography has made a notable contribution to our knowledge of structural biology. For traditional crystallography experiments, the growth of crystals with large size and high quality is crucial, and it remains one of the bottlenecks. In recent years, the successful application of serial femtosecond crystallography (SFX) provides a new choice when only numerous microcrystals can be obtained. The intense pulsed radiation of X-ray free-electron lasers (XFELs) enables the data collection of small-sized crystals, making the size of crystals no longer a limiting factor. The ultrafast pulses of XFELs can achieve 'diffraction before destruction', which effectively avoids radiation damage and realizes diffraction near physiological temperatures. More recently, the SFX has been expanded to serial crystallography (SX) that can additionally employ synchrotron radiation as the light source. In addition to the traditional ones, these techniques provide complementary opportunities for structural determination. The development of SX experiments strongly relies on the advancement of hardware including the sample delivery system, the X-ray source, and the X-ray detector. Here, in this review, we categorize the existing sample delivery systems, summarize their progress, and propose their future prospectives.
语种	英语
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/31945]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
作者单位	1.Northwestern Polytech Univ, Shenzhen Res Inst, Shenzhen, Peoples R China 2.Northwestern Polytech Univ, Sch Life Sci, 127 West Youyi Rd, Xian 710072, Shaanxi, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Appl Phys, 239 Zhangheng Rd, Shanghai 201204, Peoples R China;
推荐引用方式 GB/T 7714	Zhao, FZ,Zhang, B,Yan, EK,et al. A guide to sample delivery systems for serial crystallography[J]. FEBS JOURNAL,2019,286(22):4402-4417.
APA	Zhao, FZ.,Zhang, B.,Yan, EK.,Sun, B.,Wang, ZJ.,...&Yin, DC.(2019).A guide to sample delivery systems for serial crystallography.FEBS JOURNAL,286(22),4402-4417.
MLA	Zhao, FZ,et al."A guide to sample delivery systems for serial crystallography".FEBS JOURNAL 286.22(2019):4402-4417.