A PNA-DNA(2) Triple-Helix Molecular Switch-Based Colorimetric Sensor for Sensitive and Specific Detection of microRNAs from Cancer Cells

doi:10.1002/cbic.202000155

CORC > 宁波材料技术与工程研究所 > 中国科学院宁波材料技术与工程研究所 > 2020专题

	A PNA-DNA(2) Triple-Helix Molecular Switch-Based Colorimetric Sensor for Sensitive and Specific Detection of microRNAs from Cancer Cells
	Xu, Mengjia ; Fu, Pan ; Xing, Shu ; Zhao, Yang ; Zhao, Chao
刊名	CHEMBIOCHEM
	2020
卷号	21 期号:18 页码:2667-2675
关键词	ULTRASENSITIVE DETECTION DNA PNA AMPLIFICATION HYBRIDIZATION FLUORESCENT RECOGNITION UNIVERSAL GRAPHENE INVASION
DOI	10.1002/cbic.202000155
英文摘要	Peptide nucleic acids (PNAs), the synthetic DNA mimics that can bind to oligonucleotides to form duplexes, triplexes, and quadruplexes, could be advantageous as probes for nucleic acid sequences owing to their unique physicochemical and biochemical properties. We have found that a homopurine PNA strand could bind to two homopyrimidine DNA strands to form a PNA-DNA(2) triplex. Moreover, the cyanine dye DiSC(2)(5) could bind with high affinity to this triplex and cause a noticeable color change. On the basis of this phenomenon, we have designed a label-free colorimetric sensing platform for miRNAs from cancer cells by using a PNA-DNA(2) triple-helix molecular switch (THMS) and DiSC(2)(5). This sensing platform can detect miRNA-21 specifically with a detection limit of 0.18 nM, which is comparable to that of the THMS-mediated fluorescence sensing platform. Moreover, this colorimetric platform does not involve any chemical modification or enzymatic signal amplification, which boosts its applicability and availability at the point of care in resource-limited settings. The universality of this approach can be simply achieved by altering the sequences of the probe DNA for specific targets.
学科主题	Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
内容类型	期刊论文
源URL	[http://ir.nimte.ac.cn/handle/174433/20782]
专题	2020专题
作者单位	1.Zhao, C (corresponding author), Chinese Acad Sci, Cixi Inst Biomed Engn, Ningbo Inst Mat Technol & Engn, Ningbo 315201, Peoples R China. 2.Zhao, Y (corresponding author), Ningbo Univ, Coll Sci & Technol, Ningbo 315212, Peoples R China.
推荐引用方式 GB/T 7714	Xu, Mengjia,Fu, Pan,Xing, Shu,et al. A PNA-DNA(2) Triple-Helix Molecular Switch-Based Colorimetric Sensor for Sensitive and Specific Detection of microRNAs from Cancer Cells[J]. CHEMBIOCHEM,2020,21(18):2667-2675.
APA	Xu, Mengjia,Fu, Pan,Xing, Shu,Zhao, Yang,&Zhao, Chao.(2020).A PNA-DNA(2) Triple-Helix Molecular Switch-Based Colorimetric Sensor for Sensitive and Specific Detection of microRNAs from Cancer Cells.CHEMBIOCHEM,21(18),2667-2675.
MLA	Xu, Mengjia,et al."A PNA-DNA(2) Triple-Helix Molecular Switch-Based Colorimetric Sensor for Sensitive and Specific Detection of microRNAs from Cancer Cells".CHEMBIOCHEM 21.18(2020):2667-2675.