Dually enhanced homogenous synthesis of molybdophosphate by hybridization chain reaction and enzyme nanotags for the electrochemical bioassay of carcinoembryonic antigen

doi:10.1007/s00604-020-04342-3

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	Dually enhanced homogenous synthesis of molybdophosphate by hybridization chain reaction and enzyme nanotags for the electrochemical bioassay of carcinoembryonic antigen
	Cai, Xiaolei ; Lv, Fuhui ; Lai, Guosong ; Fu, Li ; Lin, Cheng-Te ; Yu, Aimin
刊名	MICROCHIMICA ACTA
	2020
卷号	187 期号:6
关键词	GOLD NANOPARTICLES CANCER BIOMARKER AMPLIFICATION DNA IMMUNOASSAY APTASENSOR STRATEGY
DOI	10.1007/s00604-020-04342-3
英文摘要	A magnetic bead (MB)-based sandwich biorecognition reactions is combined with a gold nanoprobe-induced homogenous synthesis of molybdophosphate to develop a novel bioassay method for the electrochemical detection of the tumor biomarker of carcinoembryonic antigen (CEA). The nanoprobe is prepared through the specific loading of numerous alkaline phosphatase (ALP)-functionalized gold nanoparticles (Au NPs) on a double-stranded DNA (dsDNA) produced by the CEA aptamer-triggered hybridization chain reaction (HCR). Both the large amounts of PO43- produced by the ALP catalytic hydrolysis of pyrophosphate and the phosphate backbones of dsDNA can react with the added MoO42- to generate electroactive molybdophosphates. So, the gold nanoprobe was used for signal tracing of the sandwich bioassay of CEA at a constructed antibody-functionalized MB platform. The sensitive electrochemical measurement of molybdophosphate produced from the quantitatively captured nanoprobes at a carbon nanotube-modified electrode (measured at about 0.12 V vs. Ag/AgCl, 3 M KCl) enabled the convenient signal transduction of the method. Due to the dually enhanced synthesis of molybdophosphate by the HCR and multi-enzyme Au NP nanotags, this method shows a wide linear range from 0.05 pg mL(-1) to 10 ng mL(-1) along with a low detection limit of 0.027 pg mL(-1). In addition, the MB-based biorecognition reaction and the homogeneous synthesis of molybdophosphate are much convenient in manipulations. These excellent performances decide the extensive application potentials of the method.
学科主题	Chemistry
内容类型	期刊论文
源URL	[http://ir.nimte.ac.cn/handle/174433/20780]
专题	2020专题
作者单位	1.Lai, GS 2.Yu, AM (corresponding author), Hubei Normal Univ, Dept Chem, Hubei Key Lab Pollutant Anal & Reuse Technol, Huangshi 435002, Hubei, Peoples R China. 3.Yu, AM (corresponding author), Swinburne Univ Technol, Fac Sci Engn & Technol, Fac Chem & Biotechnol, Hawthorn, Vic 3122, Australia.
推荐引用方式 GB/T 7714	Cai, Xiaolei,Lv, Fuhui,Lai, Guosong,et al. Dually enhanced homogenous synthesis of molybdophosphate by hybridization chain reaction and enzyme nanotags for the electrochemical bioassay of carcinoembryonic antigen[J]. MICROCHIMICA ACTA,2020,187(6).
APA	Cai, Xiaolei,Lv, Fuhui,Lai, Guosong,Fu, Li,Lin, Cheng-Te,&Yu, Aimin.(2020).Dually enhanced homogenous synthesis of molybdophosphate by hybridization chain reaction and enzyme nanotags for the electrochemical bioassay of carcinoembryonic antigen.MICROCHIMICA ACTA,187(6).
MLA	Cai, Xiaolei,et al."Dually enhanced homogenous synthesis of molybdophosphate by hybridization chain reaction and enzyme nanotags for the electrochemical bioassay of carcinoembryonic antigen".MICROCHIMICA ACTA 187.6(2020).