Microscopic visualization of cell - Cold sprayed bio-coating interfaces: An intermediate layer formed during the culturing mediates the behaviors of the cells

doi:10.1016/j.apsusc.2020.147132

CORC > 宁波材料技术与工程研究所 > 中国科学院宁波材料技术与工程研究所 > 2020专题

	Microscopic visualization of cell - Cold sprayed bio-coating interfaces: An intermediate layer formed during the culturing mediates the behaviors of the cells
	Liu, Yi ; Hou, Wenjia ; Lupoi, Rocco ; Yin, Shuo ; Huang, Jing ; Li, Hua
刊名	APPLIED SURFACE SCIENCE
	2020
卷号	529
关键词	HYDROXYAPATITE COATINGS IN-VITRO TITANIUM NANO MORPHOLOGY PARTICLES MECHANISM EVOLUTION ADHESION FILMS
DOI	10.1016/j.apsusc.2020.147132
英文摘要	Cold spraying has been proved it is a promising method for biomaterials surface modification and additive manufacturing. Fundamental understanding of the cell-biomaterial interaction is essential for design and fabrication of biomaterials for their biomedical applications. Here we report the evolutional characteristics of the interfaces between osteoblast cells and cold sprayed hydroxyapatite-titanium composite coating. The in situ observation of the interfaces was carried out by focused ion beam-scanning electron microscopy, and influence of the surface characteristics of the coatings on the behaviors of protein adsorption and cell proliferation was examined. Apparent cellular sensing, adhesion, migration, and mineral dissolution after 3 h incubation of the cells were visualized. The predominant existence of fragment particles in the coatings, one of the exceptional features of cold sprayed coating, plays vital role in regulating the cell association and cellular uptake. The presence of Ti and HA fragments at the cell-coating interface had no significant effect on cell viability and cell proliferation. After 5 days culturing, in vitro cell fusion and mineralization with the formation of an intermediate layer were evidenced on the coating, suggesting rapid regeneration and accelerated establishment of boneimplant integration. These critical phenomena occurred at different periods of culturing would facilitate understanding the influence of specific surface features of biomaterials on cell responses, and shed light on the development of manufacturing techniques for biomaterials.
学科主题	Chemistry ; Materials Science ; Physics
内容类型	期刊论文
源URL	[http://ir.nimte.ac.cn/handle/174433/20389]
专题	2020专题
作者单位	1.Liu, Y 2.Li, H (corresponding author), Chinese Acad Sci, Ningbo Inst Mat Technol & Engn, Key Lab Marine Mat & Related Technol, Zhejiang Key Lab Marine Mat & Protect Technol, Ningbo 315201, Peoples R China. 3.Li, H (corresponding author), Chinese Acad Sci, Zhejiang Engn Res Ctr Biomed Mat, Cixi Inst Biomed Engn, Ningbo Inst Mat Technol & Engn, Ningbo 315201, Peoples R China.
推荐引用方式 GB/T 7714	Liu, Yi,Hou, Wenjia,Lupoi, Rocco,et al. Microscopic visualization of cell - Cold sprayed bio-coating interfaces: An intermediate layer formed during the culturing mediates the behaviors of the cells[J]. APPLIED SURFACE SCIENCE,2020,529.
APA	Liu, Yi,Hou, Wenjia,Lupoi, Rocco,Yin, Shuo,Huang, Jing,&Li, Hua.(2020).Microscopic visualization of cell - Cold sprayed bio-coating interfaces: An intermediate layer formed during the culturing mediates the behaviors of the cells.APPLIED SURFACE SCIENCE,529.
MLA	Liu, Yi,et al."Microscopic visualization of cell - Cold sprayed bio-coating interfaces: An intermediate layer formed during the culturing mediates the behaviors of the cells".APPLIED SURFACE SCIENCE 529(2020).