Effects of Spent Drilling Fluids from Natural Gas Fields on Seed Germination and Root Development of Maize (Zea mays L.)

doi:10.3390/su13031510

	Effects of Spent Drilling Fluids from Natural Gas Fields on Seed Germination and Root Development of Maize (Zea mays L.)
	Wang, Zhe 1,2; Hao, Mingde 1
刊名	SUSTAINABILITY
	2021-02-01
卷号	13 期号:3 页码:15
关键词	spent drilling fluids land reclamation seed germination cytotoxicity chromosomal abnormalities
DOI	10.3390/su13031510
通讯作者	Hao, Mingde(mdhao@ms.iswc.ac.cn)
英文摘要	The use of drilling waste for land reclamation is a cost-effective way to improve soil fertility and to decrease landfills. However, the potential phytotoxic and cytotoxic effects of this waste on crops have not been investigated in detail. Here, we evaluated the toxicity of spent drilling fluids (SDFs) from a natural gas field using the non-target plant Zea mays L. (maize). Four different concentrations of SDFs (2%, 4%, 6%, and 8%, w/w) were used to test the toxic effects in two soils (aeolian and loessal). Different endpoints, including germination, root elongation, reactive oxygen species (ROS) accumulation, antioxidant activity, mitotic index, and chromosomal abnormalities, were used to test the effects of SDFs after four days of exposure. Higher levels (>= 6%) of SDFs inhibited seed germination and root growth, and altered the oxygen status by increasing hydrogen peroxide (H2O2) and inhibiting superoxide ion (O-2(-)) accumulation in the roots. SDFs-induced oxidative stress caused member damage, exacerbated cell injury, and reduced cell viability in the roots, compared with those untreated plants. The plants responded to high SDFs levels (>= 6%) by upregulating antioxidants such as peroxidase, superoxide dismutase ascorbate peroxidase, and proline. A reduction in the mitotic index and induction of chromosomal abnormalities in root meristematic cells were indicators of the cytotoxicity of SDFs in maize seedlings. The upregulation of antioxidants due to the change of ROS and the induction of chromosomal abnormalities were more severe in roots grown in aeolian soil than in those grown in loessal soil. The present results provide insight into the mechanism underlying the phytotoxicity and cytotoxicity of SDFs and have implications for land reclamation to minimize deleterious effects on non-target crops.
资助项目	National Natural Science Foundation of China[319611433011]
WOS研究方向	Science & Technology - Other Topics ; Environmental Sciences & Ecology
语种	英语
出版者	MDPI
WOS记录号	WOS:000615608900001
资助机构	National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.igsnrr.ac.cn/handle/311030/136122]
专题	中国科学院地理科学与资源研究所
通讯作者	Hao, Mingde
作者单位	1.Chinese Acad Sci & Minist Water Resources, Inst Soil & Water Conservat, Yangling 712100, Shaanxi, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Zhe,Hao, Mingde. Effects of Spent Drilling Fluids from Natural Gas Fields on Seed Germination and Root Development of Maize (Zea mays L.)[J]. SUSTAINABILITY,2021,13(3):15.
APA	Wang, Zhe,&Hao, Mingde.(2021).Effects of Spent Drilling Fluids from Natural Gas Fields on Seed Germination and Root Development of Maize (Zea mays L.).SUSTAINABILITY,13(3),15.
MLA	Wang, Zhe,et al."Effects of Spent Drilling Fluids from Natural Gas Fields on Seed Germination and Root Development of Maize (Zea mays L.)".SUSTAINABILITY 13.3(2021):15.