Confocal Volumetric mu XRF and Fluorescence Computed mu-Tomography Reveals Arsenic Three-Dimensional Distribution within Intact Pteris vittata Fronds

doi:10.1021/acs.est.9b03878

	Confocal Volumetric mu XRF and Fluorescence Computed mu-Tomography Reveals Arsenic Three-Dimensional Distribution within Intact Pteris vittata Fronds
	van der Ent, Antony 5,6; de Jonge, Martin D.7; Spiers, Kathryn M.8; Brueckner, Dennis 8,9,10; Montarges-Pelletier, Emmanuelle 11; Echevarria, Guillaume 6; Wan, Xiao-Ming 1,3; Lei, Mei 1,3; Mak, Rachel 4; Lovett, James H.2
刊名	ENVIRONMENTAL SCIENCE & TECHNOLOGY
	2020-01-21
卷号	54 期号:2 页码:745-757
ISSN号	0013-936X
DOI	10.1021/acs.est.9b03878
通讯作者	van der Ent, Antony(a.vanderent@uq.edu.au) ; Harris, Hugh H.(hugh.harris@adelaide.edu.au)
英文摘要	The fern Pteris vittata has been the subject of numerous studies because of its extreme arsenic hyper-accumulation characteristics. However, information on the arsenic chemical speciation and distribution across cell types within intact frozen-hydrated Pteris vittata fronds is necessary to better understand the arsenic biotransformation pathways in this unusual fern. While 2D X-ray absorption spectroscopy imaging studies show that different chemical forms of arsenic, As(III) and As(V), occur across the plant organs, depth-resolved information on arsenic distribution and chemical speciation in different cell types within tissues of Pteris vittata have not been reported. By using a combination of planar and confocal mu-X-ray fluorescence imaging and fluorescence computed mu-tomography, we reveal, in this study, the localization of arsenic in the endodermis and pericycle surrounding the vascular bundles in the rachis and the pinnules of the fern. Arsenic is also accumulated in the vascular bundles connecting into each sporangium, and in some mature sori. The use of 2D X-ray absorption near edge structure imaging allows for deciphering arsenic speciation across the tissues, revealing arsenate in the vascular bundles and arsenite in the endodermis and pericycle. This study demonstrates how different advanced synchrotron X-ray microscopy techniques can be complementary in revealing, at tissue and cellular levels, elemental distribution and chemical speciation in hyperaccumulator plants.
资助项目	Multimodal Australian ScienceS Imaging and Visualisation Environment (MASSIVE) ; Australian Research Council[DP140100176] ; Australian Research Council[DE160100429] ; French National Research Agency through the national Investissements d'avenir program[ANR-10-LABX-21] ; French National Research Agency through the national Investissements d'avenir program[ANR-14-CE04-0005] ; Program Bingwei Excellent Talent in the Institute of Geographic Sciences and Natural Resources Research, CAS
WOS关键词	X-RAY-FLUORESCENCE ; PITYROGRAMMA-CALOMELANOS ; METAL HYPERACCUMULATION ; PIXE ; SPECIATION ; ABSORPTION ; FERN ; L. ; COMPARTMENTALIZATION ; MICROTOMOGRAPHY
WOS研究方向	Engineering ; Environmental Sciences & Ecology
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000509419700011
资助机构	Multimodal Australian ScienceS Imaging and Visualisation Environment (MASSIVE) ; Australian Research Council ; French National Research Agency through the national Investissements d'avenir program ; Program Bingwei Excellent Talent in the Institute of Geographic Sciences and Natural Resources Research, CAS
内容类型	期刊论文
源URL	[http://ir.igsnrr.ac.cn/handle/311030/132398]
专题	中国科学院地理科学与资源研究所
通讯作者	van der Ent, Antony; Harris, Hugh H.
作者单位	1.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China 2.Univ Adelaide, Dept Chem, Adelaide, SA 5005, Australia 3.Chinese Acad Sci, Inst Geog Sci & Nat Resources, Res, Beijing 100101, Peoples R China 4.Univ Sydney, Sch Chem, Sydney, NSW 2006, Australia 5.Univ Queensland, Ctr Mined Land Rehabil, Sustainable Minerals Inst, St Lucia, Qld 4072, Australia 6.Univ Lorraine, UMR 1120, Lab Sols & Environm, F-54000 Nancy, France 7.ANSTO, Australian Synchrotron, 800 Blackburn Rd, Clayton, Vic 3168, Australia 8.Deutsch Elektronen Synchrotron DESY, Photon Sci, D-22607 Hamburg, Germany 9.Univ Hamburg, Dept Phys, D-20146 Hamburg, Germany 10.Ruhr Univ Bochum, Fac Chem & Biochem, D-44801 Bochum, Germany
推荐引用方式 GB/T 7714	van der Ent, Antony,de Jonge, Martin D.,Spiers, Kathryn M.,et al. Confocal Volumetric mu XRF and Fluorescence Computed mu-Tomography Reveals Arsenic Three-Dimensional Distribution within Intact Pteris vittata Fronds[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2020,54(2):745-757.
APA	van der Ent, Antony.,de Jonge, Martin D..,Spiers, Kathryn M..,Brueckner, Dennis.,Montarges-Pelletier, Emmanuelle.,...&Harris, Hugh H..(2020).Confocal Volumetric mu XRF and Fluorescence Computed mu-Tomography Reveals Arsenic Three-Dimensional Distribution within Intact Pteris vittata Fronds.ENVIRONMENTAL SCIENCE & TECHNOLOGY,54(2),745-757.
MLA	van der Ent, Antony,et al."Confocal Volumetric mu XRF and Fluorescence Computed mu-Tomography Reveals Arsenic Three-Dimensional Distribution within Intact Pteris vittata Fronds".ENVIRONMENTAL SCIENCE & TECHNOLOGY 54.2(2020):745-757.