Kinetic spectra of light-adaptation dark-adaptation and M-intermediate of BR-D96N

CORC > 西安光学精密机械研究所 > 中国科学院西安光学精密机械研究所 > 瞬态光学技术国家重点实验室

	Kinetic spectra of light-adaptation dark-adaptation and M-intermediate of BR-D96N
	Yao, BL ; Zheng, YA ; Wang, YL ; Lei, M ; Chen, GF ; Hampp, N
刊名	optics communications
	2003-03-15
卷号	218 期号:1-3 页码:125-130
关键词	bacteriorhodopsin BR-D96N photochromic kinetic spectra
ISSN号	0030-4018
通讯作者	yao baoli
英文摘要	br-d96n is a kind of genetically site-specific mutants of bacteriorhodopsin (br) with obvious photochromic effect. compared to the wild type br, the lifetime of m state of br-d96n is prolonged to several minutes so that the photochromic kinetics and the intermediates formation can be studied by the conventional spectra analysis. in the experiment, the absorption spectra of the sample at different time after light illumination are measured with spectrophotometer. by fitting and analyzing the variation of the spectra, we suppose that there are three main states in the, photochromic process, i.e., b state (light-adapted state), m state and d state (dark-adapted state). the absorption spectra of the b state, m state and d state are extracted from the experimental data based on this three-state model and the spectra at various time are fitted with the least-square method. so, the variations of population percentages of the m state, b state and d state are obtained and the m state and b state lifetimes are estimated. in another way, from the measurement of the absorption dynamics at 407 and 568 nm, the m state and b state lifetimes are also obtained by two exponential data fitting, which give coincident results with those of the spectra analysis. (c) 2003 elsevier science b.v. all rights reserved.
学科主题	光学
WOS标题词	science & technology ; physical sciences
类目[WOS]	optics
研究领域[WOS]	optics
关键词[WOS]	bacteriorhodopsin
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000181641000017
公开日期	2010-01-12
内容类型	期刊论文
源URL	[http://ir.opt.ac.cn/handle/181661/6478]
专题	西安光学精密机械研究所_瞬态光学技术国家重点实验室
作者单位	1.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt Technol, Xian 710068, Peoples R China 2.Univ Marburg, Inst Chem Phys, D-35032 Marburg, Germany
推荐引用方式 GB/T 7714	Yao, BL,Zheng, YA,Wang, YL,et al. Kinetic spectra of light-adaptation dark-adaptation and M-intermediate of BR-D96N[J]. optics communications,2003,218(1-3):125-130.
APA	Yao, BL,Zheng, YA,Wang, YL,Lei, M,Chen, GF,&Hampp, N.(2003).Kinetic spectra of light-adaptation dark-adaptation and M-intermediate of BR-D96N.optics communications,218(1-3),125-130.
MLA	Yao, BL,et al."Kinetic spectra of light-adaptation dark-adaptation and M-intermediate of BR-D96N".optics communications 218.1-3(2003):125-130.