Biodiversity and Geographic Distribution of Rhizobia Nodulating With Vigna minima

doi:10.3389/fmicb.2021.665839

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室

	Biodiversity and Geographic Distribution of Rhizobia Nodulating With Vigna minima
	Liu, Guohua 3; Liu, Xiaoling 3; Liu, Wei 4; Gao, Kangning 5; Chen, Xiaoli 1; Wang, En-Tao 2; Zhao, Zhenjun 3; Du, Wenxiao 3; Li, Yan 3,4
刊名	FRONTIERS IN MICROBIOLOGY
	2021-05-04
卷号	12 页码:11
关键词	rhizobia Bradyrhizobium diversity phylogeny Vigna distribution
ISSN号	1664-302X
DOI	10.3389/fmicb.2021.665839
通讯作者	Zhao, Zhenjun(zzjor@sina.com) ; Du, Wenxiao(duwenxiao2007@163.com) ; Li, Yan(liyan0709@hotmail.com)
英文摘要	Vigna minima is a climbing annual plant widely distributed in barren wilderness, grass land, and shrub bush of China and other countries such as Japan. However, the rhizobia nodulating with this plant has never been systematically studied. In order to reveal the biodiversity of nodulating rhizobia symbiosis with V. minima, a total of 874 rhizobium isolates were obtained from root nodules of the plant spread in 11 sampling sites of Shandong Peninsula, China, and they were designated as 41 haplotypes in the genus Bradyrhizobium based upon recA sequence analyses. By multilocus sequence analysis (MLSA) of five housekeeping genes (dnaK, glnII, gyrB, recA, and rpoB), the 41 strains representing different recA haplotypes were classified into nine defined species and nine novel genospecies. Bradyrhizobium elkanii, Bradyrhizobium ferriligni, and Bradyrhizobium pachyrhizi were the predominant and universally distributed groups. The phylogeny of symbiotic genes of nodC and nifH showed similar topology and phylogenetic relationships, in which all the representative strains were classified into two clades grouped with strains nodulating with Vigna spp., demonstrating that Vigna spp. shared common nodulating groups in the natural environment. All the representative strains formed nodules with V. minima in a nodulation test performed in green house conditions. The correlation between V. minima nodulating rhizobia and soil characteristics analyzed by CANOCO indicates that available nitrogen, total nitrogen, and organic carbon in the soil samples were the main factors affecting the distribution of rhizobia isolated in this study. This study systematically uncovered the biodiversity and distribution characteristics of V. minima nodulating rhizobia for the first time, which provided novel information for the formation of the corresponding rhizobium community.
资助项目	National Natural Science Foundation of China[31600009] ; National Natural Science Foundation of China[31800099] ; Joint Fund of Jilin Province and Chinese Academy of Sciences for High-Tech Industrialization[2019SYHZ0036] ; Key Research and Development Program of Hebei Province[19273802D] ; IPN, Mexico[SIP 2017259] ; IPN, Mexico[20180734]
WOS关键词	MULTILOCUS SEQUENCE-ANALYSIS ; SP NOV. ; SUBTROPICAL REGION ; GENETIC DIVERSITY ; ROOT-NODULES ; UNGUICULATA L. ; SALT TOLERANCE ; BRADYRHIZOBIUM ; RADIATA ; BACTERIUM
WOS研究方向	Microbiology
语种	英语
WOS记录号	WOS:000651404100001
资助机构	National Natural Science Foundation of China ; Joint Fund of Jilin Province and Chinese Academy of Sciences for High-Tech Industrialization ; Key Research and Development Program of Hebei Province ; IPN, Mexico
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/29327]
专题	海岸带生物学与生物资源利用重点实验室烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
通讯作者	Zhao, Zhenjun; Du, Wenxiao; Li, Yan
作者单位	1.Fruit Trees Work Stn Penglai, Yantai, Peoples R China 2.Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Microbiol, Mexico City, DF, Mexico 3.Yantai Univ, Coll Life Sci, Yantai, Peoples R China 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China 5.Shijiazhuang Univ, Coll Resources & Environm, Shijiazhuang, Hebei, Peoples R China
推荐引用方式 GB/T 7714	Liu, Guohua,Liu, Xiaoling,Liu, Wei,et al. Biodiversity and Geographic Distribution of Rhizobia Nodulating With Vigna minima[J]. FRONTIERS IN MICROBIOLOGY,2021,12:11.
APA	Liu, Guohua.,Liu, Xiaoling.,Liu, Wei.,Gao, Kangning.,Chen, Xiaoli.,...&Li, Yan.(2021).Biodiversity and Geographic Distribution of Rhizobia Nodulating With Vigna minima.FRONTIERS IN MICROBIOLOGY,12,11.
MLA	Liu, Guohua,et al."Biodiversity and Geographic Distribution of Rhizobia Nodulating With Vigna minima".FRONTIERS IN MICROBIOLOGY 12(2021):11.