Environmental Factors and Pollution Stresses Select Bacterial Populations in Association With Protists

doi:10.3389/fmars.2020.00659

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室

	Environmental Factors and Pollution Stresses Select Bacterial Populations in Association With Protists
	Zou, Songbao 2,3,5; Zhang, Qianqian 3; Zhang, Xiaoli 3; Dupuy, Christine 1; Gong, Jun 4,5
刊名	FRONTIERS IN MARINE SCIENCE
	2020-08-07
卷号	7 页码:19
关键词	antibiotic resistance interkingdom interaction light metal resistance protozoa warming
DOI	10.3389/fmars.2020.00659
通讯作者	Gong, Jun(gongj27@mail.sysu.edu.cn)
英文摘要	Digestion-resistant bacteria (DRB) refer to the ecological bacterial group that can be ingested, but not digested by protistan grazers, thus forming a specific type of bacteria-protist association. To test the hypothesis that the environment affects the assembly of DRB in protists, a mixotrophic ciliate, Paramecium bursaria, and a heterotrophic ciliate, Euplotes vannus, were reared at different temperatures, light conditions, and concentration gradients of antibiotic oxytetracycline and heavy metals. Community profiling indicated that the composition of DRB in both species varied significantly across the manipulated conditions, except for in P. bursaria under light/dark treatments. Clone library analysis of bacterial 16S rRNA genes showed that DRB were diverse. Pseudomonas became more abundant during the warmer treatment of P. bursaria, whereas the dominance of Pseudoalteromonas weakened and Vibrio became more abundant in E. vannus at a higher temperature. During the treatment of diel light:dark cycles, Aestuariibacter and Alteromonas were selected for in E. vannus but not Pseudoalteromonas, which was highly represented in the all-light and alldark treatments. In contrast, P. bursaria consistently hosted Nevskia, Curvibacter, and Asticcacaulis under all light conditions. There were many bacterial species co-resistant to oxytetracycline and to protistan digestion, in which Sphingomonas, Alteromonas, Aestuariibacter, Puniceicoccaceae (Verrucomicrobia), Pseudomonas, and Sulfitobacter were frequently abundant. Flectobacillus and Aestuariibacter were major lead-resistant bacteria associated with the studied protists. Acinetobacter and Hydrogenophaga were abundant in the P. bursaria treated with a high dose of mercury. Aestuariibacter was found as a dominant group of DRB in E. vannus across all cadmium treatments. In summary, this study demonstrates for the first time that environmental stress selects for bacterial populations associated with protists and that there are diverse bacterial species that not only are resistant to pollution stresses but can also survive protistan predation. This work highlights that bacteria-protists associations need to be taken into account in understanding ecological and environmental issues, such as resilience of bacterial community and function, microbial co-occurrence, and quantity and distribution of antibiotic resistant bacteria and genes.
资助项目	Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0406-4] ; Key Research Project of Frontier Science, CAS[QYZDB-SSW-DQC013-1] ; NSFC-CNRS Collaboration Project[41311130107]
WOS关键词	SP-NOV. ; HEAVY-METALS ; CHLORELLA-VARIABILIS ; COMMUNITY STRUCTURE ; SEQUENCE ALIGNMENT ; WATER-COLUMN ; FOOD QUALITY ; GEN.-NOV. ; TEMPERATURE ; DIGESTION
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
语种	英语
WOS记录号	WOS:000558705000002
资助机构	Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) ; Key Research Project of Frontier Science, CAS ; NSFC-CNRS Collaboration Project
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/28557]
专题	海岸带生物学与生物资源利用重点实验室烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室海岸带生物资源高效利用研究与发展中心
通讯作者	Gong, Jun
作者单位	1.Univ La Rochelle, CNRS, UMR 7266, Littoral Environm & Soc LIENSs, La Rochelle, France 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China 4.Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai, Peoples R China 5.Sun Yat Sen Univ, Sch Marine Sci, Zhuhai, Peoples R China
推荐引用方式 GB/T 7714	Zou, Songbao,Zhang, Qianqian,Zhang, Xiaoli,et al. Environmental Factors and Pollution Stresses Select Bacterial Populations in Association With Protists[J]. FRONTIERS IN MARINE SCIENCE,2020,7:19.
APA	Zou, Songbao,Zhang, Qianqian,Zhang, Xiaoli,Dupuy, Christine,&Gong, Jun.(2020).Environmental Factors and Pollution Stresses Select Bacterial Populations in Association With Protists.FRONTIERS IN MARINE SCIENCE,7,19.
MLA	Zou, Songbao,et al."Environmental Factors and Pollution Stresses Select Bacterial Populations in Association With Protists".FRONTIERS IN MARINE SCIENCE 7(2020):19.