Isolation and characterization of a marine bacterium Vibrio diabolicus strain L2-2 capable of biotransforming sulfonamides

doi:10.1016/j.envres.2020.109718

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Isolation and characterization of a marine bacterium Vibrio diabolicus strain L2-2 capable of biotransforming sulfonamides
	Wang, Qiaoning 1,2,3; Jiang, Yaru 2; Wang, Hongdan 1,2; Chang, Xianbo 4; Lv, Min 1,2; Chen, Lingxin 1,2,5
刊名	ENVIRONMENTAL RESEARCH
	2020-09-01
卷号	188 页码:12
关键词	Sulfonamides Vibrio diabolicus strain L2-2 Acetylation Biotransformation Arylamine N-Acetyltransferase
ISSN号	0013-9351
DOI	10.1016/j.envres.2020.109718
通讯作者	Lv, Min(mlv@yic.ac.cn) ; Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要	Sulfonamides (SAs) have attracted much attention because of their high detection rates in natural water. In this study, a marine bacterium Vibrio diabolicus strain L2-2 was isolated which could metabolize 9 SAs to a different extent. Compared with SAs and their analogs, SAs with N-oxides of heterocyclic structure were easier to be transformed to their N-4-acetylated metabolites or their isoxazole ring rearrangement isomers by strain L2-2. And, gene vdnatA and vdnatG were likely to be the key genes in SAs acetylation process, which might code Arylamine N-acetyltransferase. The biotransformation rates of sulfathiazole(STZ), sulfamonomethoxine(SMT), sulfadiazine (SDZ), sulfamethoxazole(SMX) and sulfisoxazole(SIX) could reach 29.39 +/- 5.63, 24.97 +/- 4.45, 79.41 +/- 4.05, 64.64 +/- 1.71, 32.82 +/- 4.46% in 6 days, respectively. Besides, the overall optimal conditions for SAs biotransformation were less than 100 mg/L for total SAs in neutral or weakly alkaline medium with the salinity of 10-20%o and additional nutrients like glucose, sucrose or glycerine. Furthermore, toxicity was demonstrated to be significantly reduced after biotransformation. Together, this study introduced a strategy to use V. diabolicus strain L2-2 to realize simultaneous removal and detoxification of multiple SAs in freshwater and seawater, and revealed SAs removal pathways and relevant molecular mechanism.
资助项目	National Natural Science Foundation of China[41601525] ; Key Research and Development Project of Shandong Province of China[2018GSF116011] ; Key Research and Development Project of Yantai City[2017ZH093] ; Science and Technology Development Plan of Yantai[2017ZH089] ; Taishan Scholar Project Special Funding[ts20190962] ; Project of Science and Technology Development of Shandong Province of China[2013YD21010]
WOS关键词	AMMONIA OXIDIZING BACTERIA ; ACTIVATED-SLUDGE ; SULFAMETHOXAZOLE BIODEGRADATION ; ANTIBIOTIC SULFAMETHOXAZOLE ; TRANSFORMATION PRODUCTS ; VETERINARY ANTIBIOTICS ; MICROBIAL GENOMES ; METABOLIC PATHWAY ; N-ACETYLATION ; SULFA DRUGS
WOS研究方向	Environmental Sciences & Ecology ; Public, Environmental & Occupational Health
语种	英语
WOS记录号	WOS:000564656800008
资助机构	National Natural Science Foundation of China ; Key Research and Development Project of Shandong Province of China ; Key Research and Development Project of Yantai City ; Science and Technology Development Plan of Yantai ; Taishan Scholar Project Special Funding ; Project of Science and Technology Development of Shandong Province of China
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/28508]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Lv, Min; Chen, Lingxin
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Yantai Univ, Coll Environm & Mat Engn, Yantai 264005, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Wang, Qiaoning,Jiang, Yaru,Wang, Hongdan,et al. Isolation and characterization of a marine bacterium Vibrio diabolicus strain L2-2 capable of biotransforming sulfonamides[J]. ENVIRONMENTAL RESEARCH,2020,188:12.
APA	Wang, Qiaoning,Jiang, Yaru,Wang, Hongdan,Chang, Xianbo,Lv, Min,&Chen, Lingxin.(2020).Isolation and characterization of a marine bacterium Vibrio diabolicus strain L2-2 capable of biotransforming sulfonamides.ENVIRONMENTAL RESEARCH,188,12.
MLA	Wang, Qiaoning,et al."Isolation and characterization of a marine bacterium Vibrio diabolicus strain L2-2 capable of biotransforming sulfonamides".ENVIRONMENTAL RESEARCH 188(2020):12.