Detection of hypochlorous acid fluctuation via a selective fluorescent probe in acute lung injury cells and mouse models

doi:10.1039/d0tb01969k

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	Detection of hypochlorous acid fluctuation via a selective fluorescent probe in acute lung injury cells and mouse models
	He, Na 3; Wang, Yude 1; Huang, Yan 1,2; Chen, Lingxin 2; Wang, Xiaoyan 1,2; Lv, Changjun 1; Yue, Shouwei 3
刊名	JOURNAL OF MATERIALS CHEMISTRY B
	2020-11-21
卷号	8 期号:43 页码:9899-9905
ISSN号	2050-750X
DOI	10.1039/d0tb01969k
通讯作者	Chen, Lingxin(lxchen@yic.ac.cn) ; Lv, Changjun(lucky_lcj@sina.com) ; Yue, Shouwei(shouweiy@sdu.edu.cn)
英文摘要	Acute lung injury (ALI) is a diffuse inflammatory pulmonary damage caused by excessive ROS that break the coordination of normal physiological structures and functions. Hypochlorous acid (HOCl), one kind of ROS, is a hopeful biological marker for inflammation-related diseases. Therefore, the excessive generation of HOCl might be a significant reason for oxidative injury in ALI. Herein, we developed a fluorescent probe, namely BCy-HOCl, for quantitatively monitoring and visualizing HOCl in living cells and in vivo. The probe BCy-HOCl displayed a significant fluorescence signal enhancement towards HOCl with excellent selectivity and sensitivity. The variation of HOCl in the ALI cell model and ALI mouse model was evaluated with BCy-HOCl to clarify the relationship between ALI and HOCl. Our results verified that the HOCl levels conspicuously increased with the severity of the ALI. Thus, HOCl is likely to play a crucial part in the process of ALI, which will probably provide a new strategy for its treatment.
资助项目	National Nature Science Foundation of China[81573393] ; National Nature Science Foundation of China[21804010] ; National Nature Science Foundation of China[21976209] ; Science and Technology Innovation Development Plan of Yantai of China[2020MSGY113] ; Research Initiation Fund of Binzhou Medical University[BY2019KYQD39] ; Research Initiation Fund of Binzhou Medical University[BY2020KYQD01] ; Taishan Scholar Project Special Funding[ts20190962]
WOS关键词	RESPIRATORY-DISTRESS-SYNDROME ; IMAGING BASAL ; LIVE-CELL ; MITOCHONDRIA ; HCLO ; LYSOSOME
WOS研究方向	Materials Science
语种	英语
WOS记录号	WOS:000588549200006
资助机构	National Nature Science Foundation of China ; Science and Technology Innovation Development Plan of Yantai of China ; Research Initiation Fund of Binzhou Medical University ; Taishan Scholar Project Special Funding
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/28335]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Chen, Lingxin; Lv, Changjun; Yue, Shouwei
作者单位	1.Binzhou Med Univ Hosp, Dept Resp Med, Binzhou 256603, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 3.Shandong Univ, Qilu Hosp, Cheelo Coll Med, Rehabil Ctr, Jinan 250100, Peoples R China
推荐引用方式 GB/T 7714	He, Na,Wang, Yude,Huang, Yan,et al. Detection of hypochlorous acid fluctuation via a selective fluorescent probe in acute lung injury cells and mouse models[J]. JOURNAL OF MATERIALS CHEMISTRY B,2020,8(43):9899-9905.
APA	He, Na.,Wang, Yude.,Huang, Yan.,Chen, Lingxin.,Wang, Xiaoyan.,...&Yue, Shouwei.(2020).Detection of hypochlorous acid fluctuation via a selective fluorescent probe in acute lung injury cells and mouse models.JOURNAL OF MATERIALS CHEMISTRY B,8(43),9899-9905.
MLA	He, Na,et al."Detection of hypochlorous acid fluctuation via a selective fluorescent probe in acute lung injury cells and mouse models".JOURNAL OF MATERIALS CHEMISTRY B 8.43(2020):9899-9905.