Magnetic-Field-Driven Extraction of Bioreceptors into Polymeric Membranes for Label-Free Potentiometric Biosensing

doi:10.1002/anie.202011331

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Magnetic-Field-Driven Extraction of Bioreceptors into Polymeric Membranes for Label-Free Potentiometric Biosensing
	Lv, Enguang 2,3,5; Li, Yanhong 2,3,5; Ding, Jiawang 1,2,3,4; Qin, Wei 1,2,3,4
刊名	ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
	2020-11-27
页码	6
关键词	biosensors magnetic fields peptides potentiometry
ISSN号	1433-7851
DOI	10.1002/anie.202011331
通讯作者	Ding, Jiawang(jwding@yic.ac.cn) ; Qin, Wei(wqin@yic.ac.cn)
英文摘要	We report here the concept of a magnetically controlled extraction of hydrophilic bioreceptors into polymeric membranes for bioassays. The potentiometric assay relies on the intrinsic charges of an antimicrobial peptide and its unique recognition abilities, which can eliminate the probe labeling and indicator addition. The target binding event could effectively prevent the extraction of the peptide into the polymeric membrane doped with an ion exchanger, thus resulting in a potential change. The potentiometric response properties of the peptide assembled on magnetic beads can be dynamically controlled and modulated by applying a magnetic field. Staphylococcus aureus, as a model of food-borne pathogens, was measured at levels down to 10 CFU mL(-1). Based on this sensing strategy, a potentiometric array was developed for the pattern recognition of bacteria. The proposed general platform can be used for potentiometric biosensing using other hydrophilic bioreceptors.
资助项目	National Natural Science Foundation of China[41876108] ; National Natural Science Foundation of China[21677172] ; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao)[OF2018NO01] ; Instrument Developing Project of the Chinese Academy of Sciences[Y728021021] ; National Key Research and Development Program of China[2016YFC1400700] ; Taishan Scholar Program of Shandong Province[tspd20181215] ; Taishan Scholar Program of Shandong Province[tsqn201909163] ; Key Research and Development Program of Yantai[2018ZHGY053]
WOS关键词	ION-SELECTIVE ELECTRODES ; PROTAMINE ; PROTEINS ; DESIGN
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000592466700001
资助机构	National Natural Science Foundation of China ; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao) ; Instrument Developing Project of the Chinese Academy of Sciences ; National Key Research and Development Program of China ; Taishan Scholar Program of Shandong Province ; Key Research and Development Program of Yantai
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/28311]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室烟台海岸带研究所_近岸生态与环境实验室
通讯作者	Ding, Jiawang; Qin, Wei
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China 3.YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China 4.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Lv, Enguang,Li, Yanhong,Ding, Jiawang,et al. Magnetic-Field-Driven Extraction of Bioreceptors into Polymeric Membranes for Label-Free Potentiometric Biosensing[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2020:6.
APA	Lv, Enguang,Li, Yanhong,Ding, Jiawang,&Qin, Wei.(2020).Magnetic-Field-Driven Extraction of Bioreceptors into Polymeric Membranes for Label-Free Potentiometric Biosensing.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,6.
MLA	Lv, Enguang,et al."Magnetic-Field-Driven Extraction of Bioreceptors into Polymeric Membranes for Label-Free Potentiometric Biosensing".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2020):6.