Enhancement of high-solids enzymatic hydrolysis efficiency of alkali pretreated sugarcane bagasse at low cellulase dosage by fed-batch strategy based on optimized accessory enzymes and additives

doi:10.1016/j.biortech.2019.121993

	Enhancement of high-solids enzymatic hydrolysis efficiency of alkali pretreated sugarcane bagasse at low cellulase dosage by fed-batch strategy based on optimized accessory enzymes and additives
	Xu, Chao 1,3,4,5; Zhang, Jun 1,3,4,5; Zhang, Yu 1; Guo, Ying 1; Xu, Huijuan 1; Xu, Jingliang 1,2; Wang, Zhongming 1
刊名	BIORESOURCE TECHNOLOGY
	2019-11-01
卷号	292 页码:7
关键词	Accessory enzymes Additives High-solids Enzymatic hydrolysis Lignocellulosic biomass
ISSN号	0960-8524
DOI	10.1016/j.biortech.2019.121993
通讯作者	Xu, Jingliang(xujl@zzu.edu.cn)
英文摘要	Obtaining higher amount of final sugars with low cellulase dosage has great economic benefits for the industrial biorefinery of lignocellulosic biomass. The optimization of accessory enzymes and additives were performed using single factor and orthogonal experiment firstly, after that, fed-batch strategy was applied to enhance the high-solids enzymatic hydrolysis efficiency of alkali pretreated sugarcane bagasse (SCB). A novel enzymatic hydrolysis procedure with 22% (w/v) substrate content and cellulase dosage of only 4 FPU/g dry biomass (DM) was developed, after digested for 48 h, the achieved glucose titer, yield and productivity were 122 g/L, 80% and 2.54 g L-1 h(-1), respectively. Results obtained in this study indicated a potential finding for the industrial application of lignocellulosic biomass.
资助项目	National Key Research and Development Project[2018YFB1501701] ; Guangdong Science and Technology Research Program[2017A010105018] ; Key Project of International Cooperation, Chinese Academy of Sciences[182344KYSB20160056]
WOS关键词	ETHANOL-PRODUCTION ; SACCHARIFICATION ; BIOMASS ; ACID ; LIGNOCELLULOSE ; SURFACTANTS ; TECHNOLOGY ; INHIBITION ; TWEEN-80 ; LOADINGS
WOS研究方向	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000485045400042
资助机构	National Key Research and Development Project ; Guangdong Science and Technology Research Program ; Key Project of International Cooperation, Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.giec.ac.cn/handle/344007/25679]
专题	中国科学院广州能源研究所
通讯作者	Xu, Jingliang
作者单位	1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China 2.Zhengzhou Univ, Sch Chem Engn, Zhengzhou 450001, Henan, Peoples R China 3.CAS Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China 4.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Guangdong, Peoples R China 5.Univ China Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xu, Chao,Zhang, Jun,Zhang, Yu,et al. Enhancement of high-solids enzymatic hydrolysis efficiency of alkali pretreated sugarcane bagasse at low cellulase dosage by fed-batch strategy based on optimized accessory enzymes and additives[J]. BIORESOURCE TECHNOLOGY,2019,292:7.
APA	Xu, Chao.,Zhang, Jun.,Zhang, Yu.,Guo, Ying.,Xu, Huijuan.,...&Wang, Zhongming.(2019).Enhancement of high-solids enzymatic hydrolysis efficiency of alkali pretreated sugarcane bagasse at low cellulase dosage by fed-batch strategy based on optimized accessory enzymes and additives.BIORESOURCE TECHNOLOGY,292,7.
MLA	Xu, Chao,et al."Enhancement of high-solids enzymatic hydrolysis efficiency of alkali pretreated sugarcane bagasse at low cellulase dosage by fed-batch strategy based on optimized accessory enzymes and additives".BIORESOURCE TECHNOLOGY 292(2019):7.