The responses of two genes encoding phytoene synthase (Psy) and phytoene desaturase (Pds) to nitrogen limitation and salinity up-shock with special emphasis on carotenogenesis in Dunaliella parva

doi:10.1016/j.algal.2018.03.002

	The responses of two genes encoding phytoene synthase (Psy) and phytoene desaturase (Pds) to nitrogen limitation and salinity up-shock with special emphasis on carotenogenesis in Dunaliella parva
	Shang, Changhua 2; Wang, Wen 3; Zhu, Shunni 3; Wang, Zhongming 3; Qin, Lei 3; Alam, Mohammad Asraful 3; Xie, Jun 1,4; Yuan, Zhenhong 3
刊名	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
	2018-06-01
卷号	32 页码:1-10
关键词	Dunaliella parva Salinity up-shock Nitrogen limitation Phytoene synthase Phytoene desaturase Carotenoid
ISSN号	2211-9264
DOI	10.1016/j.algal.2018.03.002
通讯作者	Shang, Changhua(shangch@ms.giec.ac.cn)
英文摘要	Green alga Dunaliella parva can adapt to environmental stresses such as salinity up-shock and nitrogen limitation which are associated with carotenoid accumulation. However, the underlying molecular basis related to the change of carotenoid content needs further study. In the present study, we cloned two carotenoid biosynthesis genes coding for phytoene synthase and phytoene desaturase. In addition, the effects of salinity up-shock and nitrogen limitation on carotenoid content and expression of these two genes were studied in D. parva FACHB-815. The results showed that both salinity up-shock and nitrogen limitation had important effect on carotenoid yield and expression of these two genes. Cloning and expression analysis of these two genes will contribute to the understanding of molecular basis in stress-induced carotenoid accumulation, which is favorable for performing metabolic and genetic engineering to improve traits of D. parva.
资助项目	Natural Science Foundation of Guangdong Province[2016A030313171] ; Natural Science Foundation of Guangdong Province[2016A030312007] ; Science and Technology Program of Guangzhou, China[201804010155] ; National Key Research and Development Program-China[2016YFB0601004] ; Pearl River S&T Nova Program of Guangzhou, China[201610010155]
WOS关键词	CHLAMYDOMONAS-REINHARDTII ; CAROTENOID ACCUMULATION ; BETA-CAROTENE ; GREEN-ALGA ; STRESS ; CHLOROPHYTA ; BIOSYNTHESIS ; EXPRESSION ; STARVATION ; BARDAWIL
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000432649800001
资助机构	Natural Science Foundation of Guangdong Province ; Science and Technology Program of Guangzhou, China ; National Key Research and Development Program-China ; Pearl River S&T Nova Program of Guangzhou, China
内容类型	期刊论文
源URL	[http://ir.giec.ac.cn/handle/344007/23617]
专题	中国科学院广州能源研究所
通讯作者	Shang, Changhua
作者单位	1.South China Agr Univ, Inst New Energy & New Mat, 483 Wushan Rd, Guangzhou 510642, Guangdong, Peoples R China 2.Guangxi Normal Univ, Minist Educ, Coll Life Sci, Key Lab Ecol Rare & Endangered Species & Environm, 1 Yanzhong Rd, Guilin 541006, Guangxi, Peoples R China 3.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, Guangdong Key Lab New & Renewable Energy Res & De, 2 Nengyuan Rd, Guangzhou 510640, Guangdong, Peoples R China 4.Minist Agr, Key Lab Biomass Energy, Key Lab Energy Plants Resource & Utilizat, Guangdong Regular Higher Educ Inst, 483 Wushan Rd, Guangzhou 510642, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Shang, Changhua,Wang, Wen,Zhu, Shunni,et al. The responses of two genes encoding phytoene synthase (Psy) and phytoene desaturase (Pds) to nitrogen limitation and salinity up-shock with special emphasis on carotenogenesis in Dunaliella parva[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2018,32:1-10.
APA	Shang, Changhua.,Wang, Wen.,Zhu, Shunni.,Wang, Zhongming.,Qin, Lei.,...&Yuan, Zhenhong.(2018).The responses of two genes encoding phytoene synthase (Psy) and phytoene desaturase (Pds) to nitrogen limitation and salinity up-shock with special emphasis on carotenogenesis in Dunaliella parva.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,32,1-10.
MLA	Shang, Changhua,et al."The responses of two genes encoding phytoene synthase (Psy) and phytoene desaturase (Pds) to nitrogen limitation and salinity up-shock with special emphasis on carotenogenesis in Dunaliella parva".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 32(2018):1-10.