Transcriptomic and proteomic analyses of genetic factors influencing adductor muscle coloration in QN Orange scallops

doi:10.1186/s12864-019-5717-y

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物资源高效利用研究与发展中心

	Transcriptomic and proteomic analyses of genetic factors influencing adductor muscle coloration in QN Orange scallops
	Song, JL ; Wang, CD
刊名	BMC GENOMICS
	2019-05-09
卷号	20 页码:363
关键词	Transcriptome Proteome Adductor muscle color QN Orange scallop
ISSN号	1471-2164
DOI	10.1186/s12864-019-5717-y
产权排序	[Song, Junlin ; Wang, Chunde] Qingdao Agr Univ, Qingdao 266109, Shandong, Peoples R China ; [Wang, Chunde] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
文献子类	Article
英文摘要	BackgroundColor polymorphism, a high-valued trait, is frequently observed in molluscan shellfish. The QN Orange scallop, a new scallop strain successively selected from the interspecific hybrids of the bay scallop (Argopecten irradians irradians) and the Peruvian scallop (Argopecten purpuratus), is distinguished from other scallops by its orange adductor muscles. In this study, to reveal the mechanisms of the formation of adductor muscle coloration in the QN Orange scallops, we compared the proteome and transcriptome of orange adductor muscles of the QN Orange and those of white adductor muscles of the Bohai Red scallop, another strain selected from the interspecific hybrids of the bay scallop and the Peruvian scallop.ResultsTranscriptomic analysis revealed 416 differentially expressed genes (DEGs) between white and orange adductor muscles, among which 216 were upregulated and 200 were downregulated. Seventy-four differentially expressed proteins (DEPs), including 36 upregulated and 38 downregulated proteins, were identified through label-free proteomics. Among the identified DEGs and DEPs, genes related to carotenoids biosynthesis including apolipophorin, and Cytochrome P450 and those related to melanin biosynthesis including tyrosinase and Ras-related protein Rab-11A were found to express at higher levels in orange adductor muscles. The high expression levels of VPS (vacuolar protein sorting) and TIF (translation initiation factor) in orange adductor muscle tissues indicated that carotenoid accumulation may be affected by proteins outside of the carotenoid pathway.ConclusionsOur results implied that the coloration of orange adductor muscles in the QN Orange scallops may be controlled by genes modulating accumulation of carotenoids and melanins. This study may provide valuable information for understanding the mechanisms and pathways underlying adductor muscle coloration in molluscan shellfish.
WOS关键词	SHELL COLOR ; PACIFIC ABALONE ; CHLAMYS-NOBILIS ; YESSO SCALLOP ; PIGMENTATION ; OYSTER
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
语种	英语
WOS记录号	WOS:000467573900005
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China [3172404] ; earmarked fund for Shandong Modern Agro-Industry Technology Research System [SDAIT-14] ; Seed Project of Shandong Province [2017LZGC009]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24942]
专题	海岸带生物资源高效利用研究与发展中心
作者单位	1.Qingdao Agr Univ, Qingdao 266109, Shandong, Peoples R China; 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Song, JL,Wang, CD. Transcriptomic and proteomic analyses of genetic factors influencing adductor muscle coloration in QN Orange scallops[J]. BMC GENOMICS,2019,20:363.
APA	Song, JL,&Wang, CD.(2019).Transcriptomic and proteomic analyses of genetic factors influencing adductor muscle coloration in QN Orange scallops.BMC GENOMICS,20,363.
MLA	Song, JL,et al."Transcriptomic and proteomic analyses of genetic factors influencing adductor muscle coloration in QN Orange scallops".BMC GENOMICS 20(2019):363.