Molecular characterization, expression, and antibacterial activity of a c-type lysozyme isolated from the manila clam, Ruditapes philippinarum

doi:10.1016/j.fsi.2018.07.036

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	Molecular characterization, expression, and antibacterial activity of a c-type lysozyme isolated from the manila clam, Ruditapes philippinarum
	Wei, Qianyu ; Mu, Changkao ; Wang, Chunlin ; Zhao, Jianmin
刊名	FISH & SHELLFISH IMMUNOLOGY
	2018-10
卷号	81 页码:502-508
关键词	Ruditapes philippinarum c-type lysozyme Tissue distribution Innate immunity
ISSN号	1050-4648
DOI	10.1016/j.fsi.2018.07.036
产权排序	[Wei, Qianyu ; Mu, Changkao ; Wang, Chunlin] Ningbo Univ, Minist Educ, Key Lab Appl Marine Biotechnol, Ningbo 315211, Zhejiang, Peoples R China ; [Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China ; [Wei, Qianyu] Ningbo Univ, Collaborat Innovat Ctr Zhejiang Marine High Effic, Ningbo 315211, Zhejiang, Peoples R China
文献子类	Article
英文摘要	Lysozyme is an antibacterial enzyme that is widely distributed in nature and plays an important role in the prevention of bacterial infections. In this study, a c-type lysozyme (designated as "RpCLYZ") was cloned and characterized from the manila clam, Ruditapes philippinarum. The full-length cDNA was 619 bp with an open reading frame (ORF) of 447 bp, and encoded a polypeptide of 148 amino acids with a calculated molecular mass of 17.0 kDa and an isoelectric point of 4.83. RpCLYZ was found to share high sequence similarity with c-type lysozymes from other invertebrates. The mRNA transcript of RpCLYZ was universally expressed in a wide range of tissues, especially in gills and mantle. Challenge with Vibrio anguillarum, significantly induced mRNA expression of RpCLYZ, which reached a maximum level 48 h after bacterial challenge. Recombinant RpCLYZ (rRpCLYZ) exhibited antibacterial activities against both Gram-positive and Gram-negative bacteria. Additionally, the optimal pH and temperature for rRpCLYZ activity were determined to be 4.5 and 20 degrees C, respectively. These results suggest that RpCLYZ participates in innate immune responses against bacterial invasion.
WOS关键词	MYTILUS-EDULIS ; CLONING ; MUSSEL
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
WOS记录号	WOS:000443665900060
资助机构	National Natural Science Foundation of China [41476126] ; Chinese Academy of Sciences [XDA11020702, XDA11020305, KZZD-EW-14] ; Youth Innovation Promotion Association CAS [2016196] ; K C Wong Magana Fund in Ningbo University
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24536]
专题	海岸带生物资源高效利用研究与发展中心烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Ningbo Univ, Minist Educ, Key Lab Appl Marine Biotechnol, Ningbo 315211, Zhejiang, Peoples R China; 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China; 3.Ningbo Univ, Collaborat Innovat Ctr Zhejiang Marine High Effic, Ningbo 315211, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Wei, Qianyu,Mu, Changkao,Wang, Chunlin,et al. Molecular characterization, expression, and antibacterial activity of a c-type lysozyme isolated from the manila clam, Ruditapes philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2018,81:502-508.
APA	Wei, Qianyu,Mu, Changkao,Wang, Chunlin,&Zhao, Jianmin.(2018).Molecular characterization, expression, and antibacterial activity of a c-type lysozyme isolated from the manila clam, Ruditapes philippinarum.FISH & SHELLFISH IMMUNOLOGY,81,502-508.
MLA	Wei, Qianyu,et al."Molecular characterization, expression, and antibacterial activity of a c-type lysozyme isolated from the manila clam, Ruditapes philippinarum".FISH & SHELLFISH IMMUNOLOGY 81(2018):502-508.