Azorhizobium caulinodans c-di-GMP phosphodiesterase Chp1 involved in motility, EPS production, and nodulation of the host plant

doi:10.1007/s00253-020-10404-6

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Azorhizobium caulinodans c-di-GMP phosphodiesterase Chp1 involved in motility, EPS production, and nodulation of the host plant
	Sun, Yu ; Liu, Yanan ; Liu, Xiaolin ; Dang, Xiaoxiao ; Dong, Xiaoyan ; Xie, Zhihong
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2020-03
卷号	104 期号:6 页码:2715-2729
关键词	CYCLIC DIGUANYLATE PSEUDOMONAS-AERUGINOSA SINORHIZOBIUM-MELILOTI SESBANIA-ROSTRATA ACETOBACTER-XYLINUM ALLOSTERIC CONTROL CRYSTAL-STRUCTURES BIOFILM FORMATION VIBRIO-CHOLERAE PAS DOMAINS
DOI	10.1007/s00253-020-10404-6
产权排序	[Sun, Yu ; Liu, Yanan ; Liu, Xiaolin ; Dang, Xiaoxiao ; Dong, Xiaoyan ; Xie, Zhihong] Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai, Peoples R China ; [Sun, Yu] Chinese Acad Sci, Northeast Inst Geog & Agroecol, Key Lab Mollisols Agroecol, Changchun, Peoples R China ; [Liu, Yanan ; Liu, Xiaolin ; Dang, Xiaoxiao] Univ Chinese Acad Sci, Beijing, Peoples R China ; [Dong, Xiaoyan ; Xie, Zhihong] Chinese Acad Sci, Ctr Ocean Mag Sci, Qingdao, Peoples R China
文献子类	Article
英文摘要	Establishment of the rhizobia-legume symbiosis is usually accompanied by hydrogen peroxide (H2O2) production by the legume host at the site of infection, a process detrimental to rhizobia. In Azorhizobium caulinodans ORS571, deletion of chp1, a gene encoding c-di-GMP phosphodiesterase, led to increased resistance against H2O2 and to elevated nodulation efficiency on its legume host Sesbania rostrata. Three domains were identified in the Chp1: a PAS domain, a degenerate GGDEF domain, and an EAL domain. An in vitro enzymatic activity assay showed that the degenerate GGDEF domain of Chp1 did not have diguanylate cyclase activity. The phosphodiesterase activity of Chp1 was attributed to its EAL domain which could hydrolyse c-di-GMP into pGpG. The PAS domain functioned as a regulatory domain by sensing oxygen. Deletion of Chp1 resulted in increased intracellular c-di-GMP level, decreased motility, increased aggregation, and increased EPS (extracellular polysaccharide) production. H2O2-sensitivity assay showed that increased EPS production could provide ORS571 with resistance against H2O2. Thus, the elevated nodulation efficiency of the increment chp1 mutant could be correlated with a protective role of EPS in the nodulation process. These data suggest that c-di-GMP may modulate the A. caulinodans-S. rostrata nodulation process by regulating the production of EPS which could protect rhizobia against H2O2.
WOS关键词	CYCLIC DIGUANYLATE ; PSEUDOMONAS-AERUGINOSA ; SINORHIZOBIUM-MELILOTI ; SESBANIA-ROSTRATA ; ACETOBACTER-XYLINUM ; ALLOSTERIC CONTROL ; CRYSTAL-STRUCTURES ; BIOFILM FORMATION ; VIBRIO-CHOLERAE ; PAS DOMAINS
语种	英语
WOS记录号	WOS:000510299300002
资助机构	Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA23050102] ; NSFC-Shandong Joint Fund Key Projects [U1806206] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31870020, 31570063]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/25095]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai, Peoples R China; 2.Chinese Acad Sci, Northeast Inst Geog & Agroecol, Key Lab Mollisols Agroecol, Changchun, Peoples R China; 3.Univ Chinese Acad Sci, Beijing, Peoples R China; 4.Chinese Acad Sci, Ctr Ocean Mag Sci, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Sun, Yu,Liu, Yanan,Liu, Xiaolin,et al. Azorhizobium caulinodans c-di-GMP phosphodiesterase Chp1 involved in motility, EPS production, and nodulation of the host plant[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2020,104(6):2715-2729.
APA	Sun, Yu,Liu, Yanan,Liu, Xiaolin,Dang, Xiaoxiao,Dong, Xiaoyan,&Xie, Zhihong.(2020).Azorhizobium caulinodans c-di-GMP phosphodiesterase Chp1 involved in motility, EPS production, and nodulation of the host plant.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,104(6),2715-2729.
MLA	Sun, Yu,et al."Azorhizobium caulinodans c-di-GMP phosphodiesterase Chp1 involved in motility, EPS production, and nodulation of the host plant".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 104.6(2020):2715-2729.