Trophic strategy of diverse methanogens across a river-to-sea gradient

doi:10.1007/s12275-019-8482-3

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Trophic strategy of diverse methanogens across a river-to-sea gradient
	Wang, BC ; Liu, FH ; Zheng, SL ; Hao, QQ
刊名	JOURNAL OF MICROBIOLOGY
	2019-06
卷号	57 期号:6 页码:470-478
关键词	trophic strategy methanogens diversity Bohai Sea (BS) river to sea gradient
ISSN号	1225-8873
DOI	10.1007/s12275-019-8482-3
产权排序	[Wang, Bingchen ; Liu, Fanghua ; Zheng, Shiling ; Hao, Qinqin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China ; [Wang, Bingchen ; Liu, Fanghua] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China ; [Wang, Bingchen ; Hao, Qinqin] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
文献子类	Article
英文摘要	Methanogens are an important biogenic source of methane, especially in estuarine waters across a river-to-sea gradient. However, the diversity and trophic strategy of methanogens in this gradient are not clear. In this study, the diversity and trophic strategy of methanogens in sediments across the Yellow River (YR) to the Bohai Sea (BS) gradient were investigated by high-throughput sequencing based on the 16S rRNA gene. The results showed that the diversity of methanogens in sediments varied from multitrophic communities in YR samples to specific methylotrophic communities in BS samples. The methanogenic community in YR samples was dominated by Methanosarcina, while that of BS samples was dominated by methylotrophic Methanococcoides. The distinct methanogens suggested that the methanogenic community of BS sediments did not originate from YR sediment input. High-throughput sequencing of the mcrA gene revealed that active Methanococcoides dominated in the BS enrichment cultures with trimethylamine as the substrate, and methylotrophic Methanolobus dominated in the YR enrichment cultures, as detected to a limited amount in in situ sediment samples. Methanosarcina were also detected in this gradient sample. Furthermore, the same species of Methanosarcina mazei, which was widely distributed, was isolated from the area across a river-to-sea gradient by the culture-dependent method. In summary, our results showed that a distribution of diverse methanogens across a river-to-sea gradient may shed light on adaption strategies and survival mechanisms in methanogens.
WOS关键词	RICE FIELD SOIL ; BOHAI SEA ; COMMUNITY ; SEDIMENTS ; MARINE ; DYNAMICS ; ARCHAEA ; STRAIN ; BACTERIAL ; REDUCTION
WOS研究方向	Microbiology
语种	英语
WOS记录号	WOS:000469228500006
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41807325, 91751112, 41573071] ; senior user project of RV KEXUE [KEXUE2018G01] ; Chinese Academy of SciencesChinese Academy of Sciences [QYZDJ-SSW-DQC015] ; Natural Science FoundationNational Natural Science Foundation of China [JQ201608, ZR2018MD011] ; Young Taishan Scholars Program of Shandong Province [tsqn20161054]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24932]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China; 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China; 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, BC,Liu, FH,Zheng, SL,et al. Trophic strategy of diverse methanogens across a river-to-sea gradient[J]. JOURNAL OF MICROBIOLOGY,2019,57(6):470-478.
APA	Wang, BC,Liu, FH,Zheng, SL,&Hao, QQ.(2019).Trophic strategy of diverse methanogens across a river-to-sea gradient.JOURNAL OF MICROBIOLOGY,57(6),470-478.
MLA	Wang, BC,et al."Trophic strategy of diverse methanogens across a river-to-sea gradient".JOURNAL OF MICROBIOLOGY 57.6(2019):470-478.