Stimulatory effect of magnetite on the syntrophic metabolism of Geobacter co-cultures: Influences of surface coating

doi:10.1016/j.gca.2018.02.009

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Stimulatory effect of magnetite on the syntrophic metabolism of Geobacter co-cultures: Influences of surface coating
	You, YS ; Zheng, SL ; Zang, HM ; Liu, F ; Liu, FH ; Liu, J
刊名	GEOCHIMICA ET COSMOCHIMICA ACTA
	2019-07-01
卷号	256 期号:SI 页码:82-96
关键词	Magnetite Direct interspecies electron transfer Surface coating Electron transfer Geobacter
ISSN号	0016-7037
DOI	10.1016/j.gca.2018.02.009
产权排序	[You, Yunshen ; Zang, Hongmei ; Liu, Feng ; Liu, Juan] Peking Univ, Coll Environm Sci & Engn, Key Lab Water & Sediment Sci, Minist Educ, Beijing 100871, Peoples R China ; [Zheng, Shiling ; Liu, Fanghua] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai, Peoples R China ; [Liu, Juan] Peking Univ, Beijing Key Lab Mineral Environm Funct, Beijing 100871, Peoples R China
文献子类	Article
英文摘要	Magnetite-mediated direct interspecies electron transfer (DIET) can facilitate syntrophic metabolism in natural microbial communities and also promote the performance of the engineered systems based on syntrophic interactions. In this study, the stimulatory effect of bare synthetic magnetite (Mt), humic acid coated magnetite, and SiO2 coated magnetite (Mt-SiO2) on DIET in defined co-cultures of Geobacter metallireducens/Geobacter sulfurreducens were studied. Magnetite coated with Aldrich humic acid (HA) and Elliott Soil humic acid (HA(ES)), respectively, were prepared, and the two kinds of humic acid influenced the ability of Mt to promote syntrophic metabolism of the co-cultures in a similar way. When weight concentration was the same, pure humic acid presented the stimulatory effect on DIET similar to bare magnetite. However, the presence of HA coating on magnetite surface caused 50% and 61%, respectively, decrease in the rates of ethanol consumption (R-e) and succinate production (R-s) in DIET processes. Pure HA in the same weight concentration as the HA coating in Mt-HA induced the similar metabolism rates as Mt-HA. In the Mt-HA mediated DIET, most electrons from ethanol metabolism were transferred to G. sulfurreducens selectively through the HA coating, and magnetite core hardly contributed to DIET processes. The SiO2 coating on magnetite resulted in 81% and 89%, respectively, decreases in R-e and R-s, mainly because the non-conductive SiO2 layer hindered electron transfer between magnetite core and bacteria. After eight-day incubation with the co-cultures, bare magnetite nanoparticles formed relatively larger and more compact aggregates with cells than Mt-HA and Mt-SiO2, due to the different surface charge between bare and coated Mt. The generation of dissolved Fe(II) and HCl-extractable Fe(II) due to microbial reduction of magnetite by G. metallireducens and vivianite formation were observed along with DIET processes in all DIET experiments. Based on these results, different pathways of electron transfer in defined co-cultures of Geobacters with bare and coated magnetite nanoparticles were proposed. The findings in this study demonstrate the significant effects of surface properties on the ability of magnetite to stimulate DIET, which needs to be considered in order to comprehensively understand the role and mechanisms of mineral-mediated DIET in natural and engineered systems. (C) 2018 Elsevier Ltd. All rights reserved.
WOS关键词	INTERSPECIES ELECTRON-TRANSFER ; HEAVY-METALS ; HUMIC-ACID ; TRANSFER MECHANISMS ; REDUCTION ; NANOPARTICLES ; MICROORGANISMS ; FE3-XTIXO4 ; REMOVAL ; FE3O4
WOS研究方向	Geochemistry & Geophysics
语种	英语
WOS记录号	WOS:000469809000007
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41472306, 41230103, 41371257, 41573071] ; National Basic Research Program of China (973 Program)National Basic Research Program of China [2014CB846001]
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24913]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Peking Univ, Coll Environm Sci & Engn, Key Lab Water & Sediment Sci, Minist Educ, Beijing 100871, Peoples R China; 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai, Peoples R China; 3.Peking Univ, Beijing Key Lab Mineral Environm Funct, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	You, YS,Zheng, SL,Zang, HM,et al. Stimulatory effect of magnetite on the syntrophic metabolism of Geobacter co-cultures: Influences of surface coating[J]. GEOCHIMICA ET COSMOCHIMICA ACTA,2019,256(SI):82-96.
APA	You, YS,Zheng, SL,Zang, HM,Liu, F,Liu, FH,&Liu, J.(2019).Stimulatory effect of magnetite on the syntrophic metabolism of Geobacter co-cultures: Influences of surface coating.GEOCHIMICA ET COSMOCHIMICA ACTA,256(SI),82-96.
MLA	You, YS,et al."Stimulatory effect of magnetite on the syntrophic metabolism of Geobacter co-cultures: Influences of surface coating".GEOCHIMICA ET COSMOCHIMICA ACTA 256.SI(2019):82-96.