Biosynthesis of phycocyanobilin in recombinant Escherichia coli

doi:10.1007/s00343-019-9060-6

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Biosynthesis of phycocyanobilin in recombinant Escherichia coli
	Ma, CB ; Li, WJ ; Ge, BS ; Lin, J ; Qin, S
刊名	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2020-03
卷号	38 期号:2 页码:529-538
关键词	combinational biosynthesis phycocyanobilin response surface methodology antioxidant
ISSN号	2096-5508
DOI	10.1007/s00343-019-9060-6
产权排序	[Ma, Chengbo ; Lin, Jian] Yantai Univ, Coll Life Sci, Yantai 264005, Peoples R China ; [Li, Wenjun ; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China ; [Ge, Baosheng] China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China ; [Li, Wenjun ; Qin, Song] Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
文献子类	Article
英文摘要	The recombinant expression of phycocyanobilin (PCB) was carried out in Escherichia coli, and the best fermentation conditions of recombinant E. coli biosynthesized PCB are optimized in the response surface methodology to improve PCB production. The recombinant PCB is extracted, isolated, and purified by methanol and chloroform extraction. Recombinant PCB is validated in UV-vis spectroscopy, high-pressure liquid chromatography, and mass spectrometry. In addition, the anti-oxidant activities of the recombinant PCB are determined. The best induction conditions that optimized by Design Expert 8.0 software include: lactose concentration 4 mmol/L, induction temperature 24.69 degrees C, induction time 4.6 h, and induction duration 13.57 h, under which the PCB expression level reached approximately 13 mg PCB/L, which is more than four times of previously reported 3 mg PCB/L. The maximum absorption peak of the recombinant PCB is located at 680 nm with a high fluorescence intensity of 470 nm. The recombinant PCB has a good ability to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals.
WOS关键词	SP PCC 6803 ; C-PHYCOCYANIN ; ANTIOXIDANT ; CHROMOPHORES ; BILIPROTEINS ; EXPRESSION
WOS研究方向	Limnology ; Oceanography
语种	英语
WOS记录号	WOS:000522834800019
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24855]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Yantai Univ, Coll Life Sci, Yantai 264005, Peoples R China; 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; 3.China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China; 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Ma, CB,Li, WJ,Ge, BS,et al. Biosynthesis of phycocyanobilin in recombinant Escherichia coli[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2020,38(2):529-538.
APA	Ma, CB,Li, WJ,Ge, BS,Lin, J,&Qin, S.(2020).Biosynthesis of phycocyanobilin in recombinant Escherichia coli.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,38(2),529-538.
MLA	Ma, CB,et al."Biosynthesis of phycocyanobilin in recombinant Escherichia coli".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 38.2(2020):529-538.