集胞藻PCC 6803中丝氨酸/苏氨酸激酶SpkC对高温胁迫的响应

doi:10.13343/j.cnki.wsxb.20170546

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	集胞藻PCC 6803中丝氨酸/苏氨酸激酶SpkC对高温胁迫的响应
	赵佳琳 ; 陈军 ; 崔玉琳 ; 于淑贤 ; 陈高 ; 秦松
刊名	微生物学报
	2018-03-07
卷号	58 期号:10 页码:1732-1742
关键词	集胞藻PCC 6803 丝氨酸/苏氨酸激酶SpkC 高温胁迫
ISSN号	0001-6209
DOI	10.13343/j.cnki.wsxb.20170546
其他题名	Response of serine/threonine protein kinase SpkC to high temperature stress in Synechocystis sp. PCC 6803
产权排序	(1)中国科学院烟台海岸带研究所 ; (2)中国科学院大学 ; (3)山东省农业科学院生物技术研究中心 ; (4)山东省作物遗传改良与生态生理重点实验室
英文摘要	Serine/threonine protein kinases play important roles to sense and transduce signal to cope with external environment. However, functions of many serine/threonine protein kinases in cyanobacteria are still unknown. [Objective] This study aims to explore whether SpkC in Synechocystis sp. PCC 6803 responses to high temperature stress. [Methods] We used the homologous recombination method to construct the spkC gene knockout mutant (ΔspkC), and then compared the growth rate, pigment contents of ΔspkC mutant and the wild strain under high temperature stress. In addition, we detected chlorophyll fluorescence under high temperature stress. Recoveries of ΔspkC and wild strain from high-temperature stress were determined through measuring the growth rate under normal temperature. [Results] Compared with wild type strain, ΔspkC mutant grew slower, and the contents of three photosynthetic pigments (chlorophyll, carotenoid and phycobilin) decreased after high temperature stress at 42 °C. The activity of photosynthetic system II in ΔspkC decreased more rapidly at 45 °C. What's more, ΔspkC strain was not able to recover after high-temperature stress for 5 days; their survival rate was significantly lower than wild-type. [Conclusion] The deletion of spkC led to severe defect response to high-temperature stress in Synechocystis sp. PCC 6803, which suggests that serine/threonine protein kinase SpkC is involved in response processes against high temperature stress.
语种	中文
CSCD记录号	CSCD:6332914
资助机构	国家自然科学基金#41406192 ; 国家自然科学基金#41176144 ; 国家自然科学基金#41376139 ; 国家重点研发计划#2016YFF0202304 ; 烟台市科技计划#2016JHZB007
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24722]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.中国科学院烟台海岸带研究所; 2.中国科学院大学; 3.山东省农业科学院生物技术研究中心; 4.山东省作物遗传改良与生态生理重点实验室
推荐引用方式 GB/T 7714	赵佳琳,陈军,崔玉琳,等. 集胞藻PCC 6803中丝氨酸/苏氨酸激酶SpkC对高温胁迫的响应[J]. 微生物学报,2018,58(10):1732-1742.
APA	赵佳琳,陈军,崔玉琳,于淑贤,陈高,&秦松.(2018).集胞藻PCC 6803中丝氨酸/苏氨酸激酶SpkC对高温胁迫的响应.微生物学报,58(10),1732-1742.
MLA	赵佳琳,et al."集胞藻PCC 6803中丝氨酸/苏氨酸激酶SpkC对高温胁迫的响应".微生物学报 58.10(2018):1732-1742.