Two macrophage migration inhibitory factors (MIFs) from the clam Ruditapes philippinarum: Molecular characterization, localization and enzymatic activities

doi:10.1016/j.fsi.2018.04.026

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中国科学院牟平海岸带环境综合试验站

	Two macrophage migration inhibitory factors (MIFs) from the clam Ruditapes philippinarum: Molecular characterization, localization and enzymatic activities
	Wang, Dan ; Yang, Dinglong ; Wang, Qing ; Zhao, Ye ; Li, Chenghua ; Wei, Qianyu ; Han, Yijing ; Zhao, Jianmin
刊名	FISH & SHELLFISH IMMUNOLOGY
	2018-07
卷号	78 页码:158-168
关键词	Manila clam Macrophage migration inhibitory factor Immune response Enzymatic activity
ISSN号	1050-4648
DOI	10.1016/j.fsi.2018.04.026
产权排序	[Wang, Dan ; Li, Chenghua ; Wei, Qianyu] Ningbo Univ, Ningbo 315211, Zhejiang, Peoples R China ; [Yang, Dinglong ; Wang, Qing ; Han, Yijing ; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China ; [Zhao, Ye] Yantai Univ, Ocean Sch, Yantai 264005, Peoples R China ; [Yang, Dinglong ; Wang, Qing ; Han, Yijing ; Zhao, Jianmin] Chinese Acad Sci, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China ; [Han, Yijing] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
文献子类	Article
英文摘要	Macrophage migration inhibitory factor (MIF) is an evolutionarily ancient cytokine-like factor and plays a critical role in both innate and adaptive immunity. In the present study, two MIFs (designed as RpMIF-1 and RpMIF-2, respectively) were identified and characterized from the clam Ruditapes philippinarum by rapid amplification of cDNA ends (RACE) approaches. The full-length cDNA of RpMIF-1 and RpMFI-2 consisted of 531 and 722 nucleotides, encoding a polypeptide of 113 and 114 amino acid residues, respectively. Multiple alignments and phylogenetic analysis revealed that both RpMIF-1 and RpMIF-2 belonged to the MIF family. The conserved catalytic-site Pro(2) for tautomerase activity was identified in the deduced amino acid sequences of RpMIFs. Both RpMIF-1 and RpMIF-2 transcripts were constitutively expressed in examined tissues of R. philippinarum with dominant expression in hepatopancreas, gills and hemocytes. Immunolocalization analysis showed that RpMIF-1 and RpMIF-2 proteins were expressed in examined tissues with the exception of adductor muscle and foot. After Vibrio anguillarum and Micrococcus luteus challenge, the mRNA expression of RpMIFs was significantly modulated in hemocytes, gills and hepatopancreas. Recombinant RpMIF-1 and RpMIF-2 proteins possessed significant tautomerase activity and oxidoreductase activity, indicating that these two proteins was perhaps involved in inflammatory responses. In summary, our results suggested that RpMIF-1 and RpMIF-2 played an important role in the innate immunity of R. philippinarum.
WOS关键词	SCALLOP CHLAMYS-FARRERI ; INNATE IMMUNE-RESPONSES ; TOLL-LIKE RECEPTOR-4 ; MARINE-INVERTEBRATES ; REGULATORY ROLE ; DANIO-RERIO ; CYTOKINE ; GENE ; EXPRESSION ; INVOLVEMENT
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
WOS记录号	WOS:000437385000019
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences [XDA11020702] ; National Natural Science Foundation of China [41576122] ; Youth Innovation Promotion Association CAS [2016196] ; K.C. Wong Magna Fund at Ningbo University
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/24444]
专题	中国科学院牟平海岸带环境综合试验站烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室海岸带生物资源高效利用研究与发展中心
作者单位	1.Ningbo Univ, Ningbo 315211, Zhejiang, Peoples R China; 2.Yantai Univ, Ocean Sch, Yantai 264005, Peoples R China; 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China; 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China;
推荐引用方式 GB/T 7714	Wang, Dan,Yang, Dinglong,Wang, Qing,et al. Two macrophage migration inhibitory factors (MIFs) from the clam Ruditapes philippinarum: Molecular characterization, localization and enzymatic activities[J]. FISH & SHELLFISH IMMUNOLOGY,2018,78:158-168.
APA	Wang, Dan.,Yang, Dinglong.,Wang, Qing.,Zhao, Ye.,Li, Chenghua.,...&Zhao, Jianmin.(2018).Two macrophage migration inhibitory factors (MIFs) from the clam Ruditapes philippinarum: Molecular characterization, localization and enzymatic activities.FISH & SHELLFISH IMMUNOLOGY,78,158-168.
MLA	Wang, Dan,et al."Two macrophage migration inhibitory factors (MIFs) from the clam Ruditapes philippinarum: Molecular characterization, localization and enzymatic activities".FISH & SHELLFISH IMMUNOLOGY 78(2018):158-168.