Label-free exonuclease I-assisted signal amplification colorimetric sensor for highly sensitive detection of kanamycin

doi:10.1016/j.foodchem.2020.128988

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	Label-free exonuclease I-assisted signal amplification colorimetric sensor for highly sensitive detection of kanamycin
	Li, Jingwen 1; Liu, Yongming 1; Lin, Hao 1; Chen, Yan 2; Liu, Zhenbo 1; Zhuang, Xuming 1; Tian, Chunyuan 1; Fu, Xiuli 1; Chen, Lingxin 3,4
刊名	FOOD CHEMISTRY
	2021-06-15
卷号	347 页码:6
关键词	Kanamycin Exonuclease I Colorimetric Protamine Gold nanoparticles
ISSN号	0308-8146
DOI	10.1016/j.foodchem.2020.128988
通讯作者	Fu, Xiuli(fxl1984.happy@163.com) ; Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要	A label-free colorimetric method based on exonuclease I (Exo I)-assisted signal amplification with protamine as a medium was developed for analysis of kanamycin. In this study, a double-stranded DNA (dsDNA) probe was tailored by manipulating an aptamer and its complementary DNA (cDNA) ensuring detection of target with high selectivity and excellent sensitivity. Herein, protamine could not only combine with negatively charged gold nanoparticles but also interaction with polyanion DNA. Upon addition of target kanamycin, the target-aptamer complex was formed and the cDNA was released. Thus, both aptamer and cDNA could be digested by Exo I, and the captured kanamycin was liberated for triggering target recycling and signal amplification. Under optimized conditions, the proposed colorimetric method realized a low detection limit of 2.8 x 10(-14) M along with a wide linear range plus excellent selectivity. Our strategy exhibited enormous potentials for fabricate various kinds of biosensors based on target-induced aptamer configuration changes.
资助项目	National Natural Science Foundation of China[21705139] ; National Natural Science Foundation of China[61801274] ; National Natural Science Foundation of China[21778047] ; Natural Science Foundation of Shandong Province in China[ZR2017BB026] ; Natural Science Foundation of Shandong Province in China[ZR2016CB25] ; Qingchuang Science and Technology Program of Shandong Province in China[2019KJF029] ; Taishan Scholar Project Special Funding[ts20190962]
WOS研究方向	Chemistry ; Food Science & Technology ; Nutrition & Dietetics
语种	英语
WOS记录号	WOS:000617924800006
资助机构	National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province in China ; Qingchuang Science and Technology Program of Shandong Province in China ; Taishan Scholar Project Special Funding
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/27402]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Fu, Xiuli; Chen, Lingxin
作者单位	1.Yantai Univ, Sch Chem & Chem Engn, Yantai 264005, Peoples R China 2.Shandong Agr & Engn Univ, Coll Resources & Environm Engn, Jinan 250100, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 4.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Li, Jingwen,Liu, Yongming,Lin, Hao,et al. Label-free exonuclease I-assisted signal amplification colorimetric sensor for highly sensitive detection of kanamycin[J]. FOOD CHEMISTRY,2021,347:6.
APA	Li, Jingwen.,Liu, Yongming.,Lin, Hao.,Chen, Yan.,Liu, Zhenbo.,...&Chen, Lingxin.(2021).Label-free exonuclease I-assisted signal amplification colorimetric sensor for highly sensitive detection of kanamycin.FOOD CHEMISTRY,347,6.
MLA	Li, Jingwen,et al."Label-free exonuclease I-assisted signal amplification colorimetric sensor for highly sensitive detection of kanamycin".FOOD CHEMISTRY 347(2021):6.