Seagrass (Zostera marina) promotes nitrification potential and selects specific ammonia oxidizers in coastal sediments

doi:10.1007/s11368-021-02951-w

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Seagrass (Zostera marina) promotes nitrification potential and selects specific ammonia oxidizers in coastal sediments
	Lin, Xianbiao 4,5; Zheng, Pengfei 2,3; Zou, Songbao 2,3; Sun, Feifei 2; Zhang, Xiaoli 1,2; Gong, Jun 4,5
刊名	JOURNAL OF SOILS AND SEDIMENTS
	2021-04-19
页码	15
关键词	Seagrass meadow Potential nitrification rates Relative importance Gene copy number Specific lineage
ISSN号	1439-0108
DOI	10.1007/s11368-021-02951-w
通讯作者	Zhang, Xiaoli(xlzhang@yic.ac.cn) ; Gong, Jun(gongj27@mail.sysu.edu.cn)
英文摘要	Purpose Seagrasses accelerate sedimentation, release oxygen and organic matter through their roots, and compete with ammonia oxidizers for ammonia/ammonium in surface sediments and overlying water, all of which can influence benthic aerobic nitrification. To understand the effects of seagrass vegetation on benthic nitrification, the heterogeneity of nitrification activities and functional microbial communities between seagrass-vegetated and adjacent bare sediments was investigated. Materials and methods Surface (0-5 cm) sediments were sampled from a Zostera marina-colonized coastal lagoon in northern China. The potential nitrification rates (PNR) and relative contributions of ammonia-oxidizing bacteria (PNRaob) and ammonia-oxidizing archaea (PNRaoa) were determined based on the sediment slurry incubation with kanamycin inhibition method. The abundances and community compositions of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) were determined using qPCR, clone library, and high-throughput sequencing. Results and discussion The total PNR (PNRtotal) in the seagrass-colonized sediments were significant higher than those in the bare sediments (P < 0.05), to which PNRaob contributed 59.7% and 55.8% in vegetated and unvegetated sediments, respectively. The concentrations of chlorophyll a and dissolved inorganic nitrogen were key factors determining benthic PNR. AOB outnumbered AOA by one order of magnitude by targeting amoA gene abundance, and no significant difference (P > 0.05) in amoA gene abundance and diversity was observed between the two habitats. Most AOB genotypes were affiliated to Nitrosomonas, of which NL7 was selectively enriched in the vegetated region, while N. cryotolerans prevailed in the bare. Two distinct AOA groups Crenarchaeota 1.1b and Crenarchaeota 1.1a dominated in vegetated and unvegetated sediments, respectively. Dissolved oxygen of overlying water and TOC:TN of sediment significantly influenced AOB community, while AOA community was strongly driven by nitrate and metal iron in sediments. Conclusions Seagrass vegetation substantially enhances nitrification potential and selects specific ammonia oxidizers in coastal sediments.
资助项目	Natural Science Foundation of China[41676154] ; Natural Science Foundation of China[41976115] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA23050303] ; Key Research and Development project of Yantai[2017ZH095] ; Key Research Program of Frontier Sciences of CAS[QYZDB-SSW-DQC013] ; Key Research Program of Frontier Sciences of CAS[QYZDB-SSWDQC041] ; China Postdoctoral Science Foundation[2019M653151] ; Fundamental Research Funds for the Central Universities[19lgpy93] ; Fundamental Research Funds for the Central Universities[181gzd07]
WOS研究方向	Environmental Sciences & Ecology ; Agriculture
语种	英语
WOS记录号	WOS:000641218700003
资助机构	Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Key Research and Development project of Yantai ; Key Research Program of Frontier Sciences of CAS ; China Postdoctoral Science Foundation ; Fundamental Research Funds for the Central Universities
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/27299]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
通讯作者	Zhang, Xiaoli; Gong, Jun
作者单位	1.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remedia, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100085, Peoples R China 4.Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China 5.Sun Yat Sen Univ, Sch Marine Sci, Zhuhai 519082, Peoples R China
推荐引用方式 GB/T 7714	Lin, Xianbiao,Zheng, Pengfei,Zou, Songbao,et al. Seagrass (Zostera marina) promotes nitrification potential and selects specific ammonia oxidizers in coastal sediments[J]. JOURNAL OF SOILS AND SEDIMENTS,2021:15.
APA	Lin, Xianbiao,Zheng, Pengfei,Zou, Songbao,Sun, Feifei,Zhang, Xiaoli,&Gong, Jun.(2021).Seagrass (Zostera marina) promotes nitrification potential and selects specific ammonia oxidizers in coastal sediments.JOURNAL OF SOILS AND SEDIMENTS,15.
MLA	Lin, Xianbiao,et al."Seagrass (Zostera marina) promotes nitrification potential and selects specific ammonia oxidizers in coastal sediments".JOURNAL OF SOILS AND SEDIMENTS (2021):15.