Runx2/Osterix and Zinc Uptake Synergize to Orchestrate Osteogenic Differentiation and Citrate Containing Bone Apatite Formation | |
Fu, Xuekun2; Li, Yunyan1; Huang,Tongling2; Yu, Zhiwu1; Ma, Kun1; Yang, Meng2; Liu, Qingli2; Pan, Haobo2; Wang, Huaiyu2; Wang, Junfeng1 | |
刊名 | Advanced Science |
2018-04 | |
英文摘要 | Citrate is essential to biomineralization of the bone especially as an integral part of apatite nanocomposite. Citrate precipitate of apatite is hypothesized to be derived from mesenchymal stem/stromal cells (MSCs) upon differentiation into mature osteoblasts. Based on 13C-labeled signals identified by solid-state multinuclear magnetic resonance analysis, boosted mitochondrial activity and carbon-source replenishment of tricarboxylic acid cycle intermediates coordinate to feed forward mitochondrial anabolism and deposition of citrate. Moreover, zinc (Zn2+) is identified playing dual functions: (i) Zn2+ influx is influenced by ZIP1 which is regulated by Runx2 and Osterix to form a zinc-Runx2/Osterix-ZIP1 regulation axis promoting osteogenic differentiation; (ii) Zn2+ enhances citrate accumulation and deposition in bone apatite. Furthermore, age-related bone loss is associated with Zn2+ and citrate homeostasis; whereas, restoration of Zn2+ uptake alleviates age-associated declining osteogenic capacity and amount of citrate deposition. Together, these results indicate that citrate is not only a key metabolic intermediate meeting the emerging energy demand of differentiating MSCs but also participates in extracellular matrix mineralization, providing mechanistic insight into Zn2+ homeostasis and bone formation. |
URL标识 | 查看原文 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/43274] |
专题 | 合肥物质科学研究院_中科院强磁场科学中心 |
通讯作者 | Wang, Junfeng; Guan, Min |
作者单位 | 1.Chinese Acad Sci, Anhui Univ, Inst Phys Sci & Informat Technol, High Magnet Field Lab,Key Lab High Magnet Field &, Hefei 230031, Anhui, Peoples R China. 2.Chinese Acad Sci, Ctr Human Tissues & Organs Degenerat, Shenzhen Inst Adv Technol, Inst Biomed & Biotechnol, Shenzhen 518055, Guangdong, Peoples R China. |
推荐引用方式 GB/T 7714 | Fu, Xuekun,Li, Yunyan,Huang,Tongling,et al. Runx2/Osterix and Zinc Uptake Synergize to Orchestrate Osteogenic Differentiation and Citrate Containing Bone Apatite Formation[J]. Advanced Science,2018. |
APA | Fu, Xuekun.,Li, Yunyan.,Huang,Tongling.,Yu, Zhiwu.,Ma, Kun.,...&Guan, Min.(2018).Runx2/Osterix and Zinc Uptake Synergize to Orchestrate Osteogenic Differentiation and Citrate Containing Bone Apatite Formation.Advanced Science. |
MLA | Fu, Xuekun,et al."Runx2/Osterix and Zinc Uptake Synergize to Orchestrate Osteogenic Differentiation and Citrate Containing Bone Apatite Formation".Advanced Science (2018). |
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