Gasotransmitter Regulation of Phosphatase Activity in Live Cells Studied by Three-Channel Imaging Correlation | |
Ou, Pan1,3; Zhang, Ruilong1,3; Liu, Zhengjie1,3; Tian, Xiaohe1,3; Han, Guangmei2; Liu, Bianhua2; Hu, Zhangjun4; Zhang, Zhongping1,2,3 | |
刊名 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION |
2019-02-18 | |
卷号 | 58期号:8页码:2261-2265 |
关键词 | enzyme regulation fluorescent probes gasotransmitters imaging agents phosphatase |
ISSN号 | 1433-7851 |
DOI | 10.1002/anie.201811391 |
英文摘要 | Enzyme activity in live cells is dynamically regulated by small-molecule transmitters for maintaining normal physiological functions. A few probes have been devised to measure intracellular enzyme activities by fluorescent imaging, but the study of the regulation of enzyme activity via gasotransmitters in situ remains a long-standing challenge. Herein, we report a three-channel imaging correlation by a single dual-reactive fluorescent probe to measure the dependence of phosphatase activity on the H2S level in cells. The two sites of the probe reactive to H2S and phosphatase individually produce blue and green fluorescent responses, respectively, and resonance energy transfer can be triggered by their coexistence. Fluorescent analysis based on the three-channel imaging correlation shows that cells have an ideal level of H2S to promote phosphatase activity up to its maximum. Significantly, a slight deviation from this H2S level leads to a sharp decrease of phosphatase activity. The discovery further strengthens our understanding of the importance of H2S in cellular signaling and in various human diseases. |
资助项目 | National Basic Research Program of China[2015CB932002] ; National Natural Science Foundation of China[21335006] ; National Natural Science Foundation of China[21475135] ; National Natural Science Foundation of China[21375131] ; National Natural Science Foundation of China[21775001] ; National Natural Science Foundation of China[21705001] ; National Natural Science Foundation of China[21602003] ; Anhui Provincial Natural Science Foundation of China[1708085MC68] ; Anhui Provincial Natural Science Foundation of China[1808085MB32] ; Hefei Physical Science Center Foundation[2017FXZY001] |
WOS关键词 | SINGLE FLUORESCENT-PROBE ; HYDROGEN-SULFIDE ; POLYSULFIDES ; ACTIVATION ; MOLECULE ; SYNTHASE ; ENZYMES ; DESIGN |
WOS研究方向 | Chemistry |
语种 | 英语 |
出版者 | WILEY-V C H VERLAG GMBH |
WOS记录号 | WOS:000458417700011 |
资助机构 | National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Basic Research Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation ; Hefei Physical Science Center Foundation |
内容类型 | 期刊论文 |
源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/41800] |
专题 | 合肥物质科学研究院_中科院合肥智能机械研究所 |
通讯作者 | Zhang, Ruilong; Zhang, Zhongping |
作者单位 | 1.Anhui Univ, Inst Phys Sci & Informat Technol, Hefei 230601, Anhui, Peoples R China 2.Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Anhui, Peoples R China 3.Anhui Univ, Sch Chem & Chem Engn, Hefei 230601, Anhui, Peoples R China 4.Linkoping Univ, Dept Phys Chem & Biol, S-58183 Linkoping, Sweden |
推荐引用方式 GB/T 7714 | Ou, Pan,Zhang, Ruilong,Liu, Zhengjie,et al. Gasotransmitter Regulation of Phosphatase Activity in Live Cells Studied by Three-Channel Imaging Correlation[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2019,58(8):2261-2265. |
APA | Ou, Pan.,Zhang, Ruilong.,Liu, Zhengjie.,Tian, Xiaohe.,Han, Guangmei.,...&Zhang, Zhongping.(2019).Gasotransmitter Regulation of Phosphatase Activity in Live Cells Studied by Three-Channel Imaging Correlation.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,58(8),2261-2265. |
MLA | Ou, Pan,et al."Gasotransmitter Regulation of Phosphatase Activity in Live Cells Studied by Three-Channel Imaging Correlation".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 58.8(2019):2261-2265. |
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