Composition-dependent membrane disruption by the proapoptotic protein PB1F2 from HK97 influenza A virus

doi:10.1002/1873-3468.13172

CORC > 合肥物质科学研究院 > 中国科学院合肥物质科学研究院 > 中科院强磁场科学中心

	Composition-dependent membrane disruption by the proapoptotic protein PB1F2 from HK97 influenza A virus
	Wang, Yujuan 1,3; Yang, Jing 1,3; Wang, Jiarong 1,3; Zhu, Lei 1,3; Wang, Junfeng 1,2,3
刊名	FEBS LETTERS
	2018-08-01
卷号	592 期号:15 页码:2572-2581
关键词	influenza A virus membrane disruption micelle-like lipid assembly NMR PB1F2
ISSN号	1873-3468
DOI	10.1002/1873-3468.13172
通讯作者	Wang, Yujuan(Yjwang@hmfl.ac.cn) ; Wang, Junfeng(Junfeng@hmfl.ac.cn)
英文摘要	PBIF2 is a proapoptotic protein encoded by an alternative reading frame in the influenza A virus. Its accumulation accelerates mitochondrial fragmentation by decreasing the mitochondrial membrane potential following translocation into the mitochondrial inner membrane space, but the mechanistic underpinnings remain unclear. Herein, the PB1F2 from HK97 was expressed and purified in soluble form. The interaction between PBIF2 and the mitochondrial membrane were investigated using three membrane mimics, liposomes, bicelles, and nanodiscs. We show that the interactions between PBIF2 and membrane mimics depend on lipid type and are time- and dose-dependent. The primary membrane target of PBIF2 is phosphatidylcholine, the lipid that forms the major component of mitochondria! inner membranes. PBIF2 disrupts the integrity of lipid membranes by forming micelle-like PBIF2-lipid assemblies.
资助项目	National Natural Science Foundation of China[31200579] ; National Natural Science Foundation of China[21473210] ; National Natural Science Foundation of China[21502196] ; National Natural Science Foundation of China[U1532269] ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology[2016FXCX005]
WOS关键词	PB1-F2 PROTEIN ; MITOCHONDRIAL-MEMBRANE ; PORE FORMATION ; DAMAGE ; OLIGOMERS ; DOMAIN ; BETA
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
语种	英语
出版者	WILEY
WOS记录号	WOS:000441436400007
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology ; Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/37694]
专题	合肥物质科学研究院_中科院强磁场科学中心
通讯作者	Wang, Yujuan; Wang, Junfeng
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, High Field Magnet Lab, Hefei, Anhui, Peoples R China 2.Anhui Univ, Inst Phys Sci & Informat Technol, Hefei, Anhui, Peoples R China 3.Chinese Acad Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yujuan,Yang, Jing,Wang, Jiarong,et al. Composition-dependent membrane disruption by the proapoptotic protein PB1F2 from HK97 influenza A virus[J]. FEBS LETTERS,2018,592(15):2572-2581.
APA	Wang, Yujuan,Yang, Jing,Wang, Jiarong,Zhu, Lei,&Wang, Junfeng.(2018).Composition-dependent membrane disruption by the proapoptotic protein PB1F2 from HK97 influenza A virus.FEBS LETTERS,592(15),2572-2581.
MLA	Wang, Yujuan,et al."Composition-dependent membrane disruption by the proapoptotic protein PB1F2 from HK97 influenza A virus".FEBS LETTERS 592.15(2018):2572-2581.