Circular RNA hsa_circ_0000700 promotes cell proliferation and migration in Esophageal Squamous Cell Carcinoma by sponging miR-1229

	Circular RNA hsa_circ_0000700 promotes cell proliferation and migration in Esophageal Squamous Cell Carcinoma by sponging miR-1229
	Fang, Jun 1; Ji, Wen Hao 1; Wang, Fang Zheng 1,2; Xie, Tie Ming 3; Wang, Lei 1,2; Fu, Zhen Fu 1,2; Wang, Zhun 1; Yan, Feng Qin 1,2; Shen, Qi Liang 1; Ye, Zhi Min 1,2
刊名	JOURNAL OF CANCER
	2021
卷号	12
关键词	esophageal squamous cell cancer hsa_circ_0000700 hsa_miR-1229 cell proliferation cell migration
ISSN号	1837-9664
通讯作者	Ye, Zhi Min(yezm@zjcc.org.cn)
英文摘要	Accumulating evidence has demonstrated that circular RNAs (circRNAs) are involved in the pathogenesis of cancer, including that of esophageal squamous cell carcinoma (ESCC). The current study aimed to investigate the role of hsa_circ_0000700 in ESCC. hsa_circ_0000700, miR-1229, and related functional gene expression was measured by RT-qPCR. To characterize the functions of hsa_circ_0000700 and miR-1229, ESCC cells were infected with hsa_circ_0000700-specific siRNA, miR-1229 mimics, and an inhibitor alone or in combination. Cell Counting Kit-8 (CCK8), colony formation, EdU, flow cytometry, and Transwell assays were employed to evaluate cell proliferation, apoptosis, and migration. Luciferase reporter and RNA immunoprecipitation assays were used to confirm the targeting relationship between hsa_circ_0000700 and miR-1229. Finally, a competing endogenous RNAs (ceRNA) network was built for hsa_circ_0000700, and miR-1229 targets were analyzed by bioinformatics. circ_0000700 expression was significantly upregulated in ESCC cell lines. Actinomycin D and RNase R treatment confirmed that circ_0000700 was more stable than its linear CDH9 mRNA form. Moreover, a cytoplasmic and nuclear fractionation assay suggested that circ_0000700 was mainly distributed in the cytoplasm of ECA-109 and TE-1 cells. In vitro, the proliferative and migratory capacities of ECA-109 and TE-1 cells were inhibited by knocking down circ_0000700 expression. Additionally, miR-1229 silencing reversed the circ_0000700-specific siRNA-induced attenuation of malignant phenotypes. Mechanistically, circ_0000700 was identified as a sponge of miR-1229 and could activate PRRG4, REEP5, and PSMB5 indirectly to promote ESCC progression. In summary, our results suggest that hsa_circ_0000700 functions as an oncogenic factor by sponging miR-1229 in ESCC.
资助项目	Zhejiang Provincial Natural Science Foundation of China[LSY19A010001] ; Zhejiang Provincial Natural Science Foundation of China[LY19H160004] ; Zhejiang Provincial Natural Science Foundation of China[LY18H160031] ; Zhejiang Medical and Health Science and Technology Project[2015122748] ; Zhejiang Medical and Health Science and Technology Project[2018260661] ; Zhejiang Medical and Health Science and Technology Project[2019326095] ; Zhejiang Medical and Health Science and Technology Project[2019328882]
WOS研究方向	Oncology
语种	英语
出版者	IVYSPRING INT PUBL
WOS记录号	WOS:000629081300013
资助机构	Zhejiang Provincial Natural Science Foundation of China ; Zhejiang Medical and Health Science and Technology Project
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/121221]
专题	中国科学院合肥物质科学研究院
通讯作者	Ye, Zhi Min
作者单位	1.Chinese Acad Sci, Inst Basic Med & Canc IBMC, Zhejiang Canc Hosp, Dept Radiotherapy,Canc Hosp,Univ Chinese Acad Sci, Hangzhou 310022, Zhejiang, Peoples R China 2.Key Lab Head & Neck Canc Translat Res Zhejiang Pr, 1 Banshan East Rd, Hangzhou 310022, Zhejiang, Peoples R China 3.Chinese Acad Sci, Inst Basic Med & Canc IBMC, Zhejiang Canc Hosp, Dept Radiol,Canc Hosp,Univ Chinese Acad Sci, Hangzhou 310022, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Fang, Jun,Ji, Wen Hao,Wang, Fang Zheng,et al. Circular RNA hsa_circ_0000700 promotes cell proliferation and migration in Esophageal Squamous Cell Carcinoma by sponging miR-1229[J]. JOURNAL OF CANCER,2021,12.
APA	Fang, Jun.,Ji, Wen Hao.,Wang, Fang Zheng.,Xie, Tie Ming.,Wang, Lei.,...&Ye, Zhi Min.(2021).Circular RNA hsa_circ_0000700 promotes cell proliferation and migration in Esophageal Squamous Cell Carcinoma by sponging miR-1229.JOURNAL OF CANCER,12.
MLA	Fang, Jun,et al."Circular RNA hsa_circ_0000700 promotes cell proliferation and migration in Esophageal Squamous Cell Carcinoma by sponging miR-1229".JOURNAL OF CANCER 12(2021).