Rapid One-Pot Detection of SARS-CoV-2 Based on a Lateral Flow Assay in Clinical Samples

doi:10.1021/acs.analchem.0c05059

	Rapid One-Pot Detection of SARS-CoV-2 Based on a Lateral Flow Assay in Clinical Samples
	Zhang, Chao 1; Zheng, Tingting 1; Wang, Hua 2; Chen, Wei 3; Huang, Xiaoye 3; Liang, Jianqi 3; Qiu, Liping 4; Han, Da 1; Tan, Weihong 1,4,5
刊名	ANALYTICAL CHEMISTRY
	2021-02-23
卷号	93
ISSN号	0003-2700
DOI	10.1021/acs.analchem.0c05059
通讯作者	Han, Da(dahan@sjtu.edu.cn) ; Tan, Weihong(tan@hnu.edu.cn)
英文摘要	Rapid tests for pathogen identification and spread assessment are critical for infectious disease control and prevention. The control of viral outbreaks requires a nucleic acid diagnostic test that is sensitive and simple and delivers fast and reliable results. Here, we report a one-pot direct reverse transcript loop-mediated isothermal amplification (RT-LAMP) assay of SARS-CoV-2 based on a lateral flow assay in clinical samples. The entire contiguous sample-to-answer workflow takes less than 40 min from a clinical swab sample to a diagnostic result without professional instruments and technicians. The assay achieved an accuracy of 100% in 12 synthetic and 12 clinical samples compared to the data from PCR-based assays. We anticipate that our method will provide a universal platform for rapid and point-of-care detection of emerging infectious diseases.
资助项目	COVID-19 specialized grant from the Ministry of Science and Technology of China[2020YFC0842800] ; COVID-19 specialized grant from Shanghai Jiao Tong University ; COVID-19 specialized grant from Hunan province[2020SK3008] ; National Natural Science Foundation of China[91953000] ; National Natural Science Foundation of China[21974087]
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000623041000001
资助机构	COVID-19 specialized grant from the Ministry of Science and Technology of China ; COVID-19 specialized grant from Shanghai Jiao Tong University ; COVID-19 specialized grant from Hunan province ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/120856]
专题	中国科学院合肥物质科学研究院
通讯作者	Han, Da; Tan, Weihong
作者单位	1.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med,Shanghai Key Lab Nucle Acid Chem & Nanome, Inst Mol Med IMM,State Key Lab Oncogenes & Relate, Shanghai 200127, Peoples R China 2.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Dept Lab Med, Shanghai 200127, Peoples R China 3.Cent Hosp Loudi, Clin Lab, Loudi 417099, Hunan, Peoples R China 4.Hunan Univ, Coll Chem & Chem Engn, Aptamer Engn Ctr Hunan Prov, Mol Sci & Biomed Lab MBL,State Key Lab Chemo Bios, Changsha 410082, Hunan, Peoples R China 5.Univ Chinese Acad Sci, Chinese Acad Sci, Inst Canc & Basic Med ICBM, Canc Hosp, Hangzhou 310018, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Chao,Zheng, Tingting,Wang, Hua,et al. Rapid One-Pot Detection of SARS-CoV-2 Based on a Lateral Flow Assay in Clinical Samples[J]. ANALYTICAL CHEMISTRY,2021,93.
APA	Zhang, Chao.,Zheng, Tingting.,Wang, Hua.,Chen, Wei.,Huang, Xiaoye.,...&Tan, Weihong.(2021).Rapid One-Pot Detection of SARS-CoV-2 Based on a Lateral Flow Assay in Clinical Samples.ANALYTICAL CHEMISTRY,93.
MLA	Zhang, Chao,et al."Rapid One-Pot Detection of SARS-CoV-2 Based on a Lateral Flow Assay in Clinical Samples".ANALYTICAL CHEMISTRY 93(2021).