Integration of Transcriptomics and Metabolomics Reveals the Antitumor Mechanism Underlying Shikonin in Colon Cancer

doi:10.3389/fphar.2020.544647

	Integration of Transcriptomics and Metabolomics Reveals the Antitumor Mechanism Underlying Shikonin in Colon Cancer
	Chen, Yang 2; Gao, Yun 1,3,4; Yi, Xiaojiao 2; Zhang, Jinghui 2; Chen, Zhongjian 1,3,4; Wu, Yongjiang 2
刊名	FRONTIERS IN PHARMACOLOGY
	2020-10-22
卷号	11
关键词	transcriptomics metabolomics shikonin colon cancer purine metabolism
ISSN号	1663-9812
DOI	10.3389/fphar.2020.544647
通讯作者	Chen, Zhongjian(chenzj@zjcc.org.cn) ; Wu, Yongjiang(yjwu@zju.edu.cn)
英文摘要	Colorectal cancer is a common malignancy occurring in the digestive system, which is the third common cause of cancer mortality in developed countries. Shikonin, a naphthoquinone compound extracted from the root of Lithospermum erythrorhizon, is extensively reported to exert antitumor activity against various types of cancer. However, the systematic effect of shikonin in colon cancer remains poorly understood. In the present study, we evaluated the antitumor activity of shikonin in human colon cancer cells and the therapeutic effect on a xenograft mouse model. Transcriptomics and metabolomics were further integrated to provide a systematic perspective of the shikonin-induced antitumor mechanism. The results demonstrated that shikonin had a remarkable antitumor potency both in vitro and in vivo. Moreover, metabolic pathways, including the purine metabolism, amino acid metabolism, and glycerophospholipid metabolism, were perturbed and subsequently led to cell cycle arrest in the G2/M phase. In particular, the disturbance of purine metabolism may account for the major mechanism resulting from shikonin antitumor activity.
资助项目	National Great New Drug Research and Development project[2018ZX09201010] ; National Natural Science Foundation of China[81302840]
WOS关键词	NUCLEOTIDE-METABOLISM ; CELL-LINE ; PURINE ; NECROPTOSIS ; APOPTOSIS ; ADENOSINE ; PAICS
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000592411700001
资助机构	National Great New Drug Research and Development project ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/105323]
专题	中国科学院合肥物质科学研究院
通讯作者	Chen, Zhongjian; Wu, Yongjiang
作者单位	1.Univ Chinese Acad Sci, Canc Hosp, Hangzhou, Peoples R China 2.Zhejiang Univ, Coll Pharmaceut Sci, Hangzhou, Peoples R China 3.Chinese Acad Sci, Inst Canc & Basic Med ICBM, Hangzhou, Peoples R China 4.Zhejiang Canc Hosp, Hangzhou, Peoples R China
推荐引用方式 GB/T 7714	Chen, Yang,Gao, Yun,Yi, Xiaojiao,et al. Integration of Transcriptomics and Metabolomics Reveals the Antitumor Mechanism Underlying Shikonin in Colon Cancer[J]. FRONTIERS IN PHARMACOLOGY,2020,11.
APA	Chen, Yang,Gao, Yun,Yi, Xiaojiao,Zhang, Jinghui,Chen, Zhongjian,&Wu, Yongjiang.(2020).Integration of Transcriptomics and Metabolomics Reveals the Antitumor Mechanism Underlying Shikonin in Colon Cancer.FRONTIERS IN PHARMACOLOGY,11.
MLA	Chen, Yang,et al."Integration of Transcriptomics and Metabolomics Reveals the Antitumor Mechanism Underlying Shikonin in Colon Cancer".FRONTIERS IN PHARMACOLOGY 11(2020).