Peritoneal metastatic gastric carcinoma cells exhibit more malignant behavior when co-cultured with HMrSV5 cells

doi:10.18632/aging.102803

	Peritoneal metastatic gastric carcinoma cells exhibit more malignant behavior when co-cultured with HMrSV5 cells
	Wang, Lijing 2,3; Xu, Zhiyuan 1,3; Hu, Can 4; Chen, Shangqi 4; Du, Yian 1,3; Huang, Ling 1,3; Shi, Chengwei 4; Mo, Shaowei 4; Cheng, Xiangdong 1,3
刊名	AGING-US
	2020-02-29
卷号	12
关键词	peritoneal metastasis gastric cancer NUGC-4 cells
ISSN号	1945-4589
DOI	10.18632/aging.102803
通讯作者	Cheng, Xiangdong(chengxd@zjcc.org.cn)
英文摘要	Metastasis and recurrence are major causes of death in gastric cancer patients. Because there are no obvious clinical symptoms during the early stages of metastasis, we sought to isolate highly invasive metastatic gastric cancer cells for future drug screening. We first established a mouse model to observe gastric cancer metastasis in vivo. The incidence of peritoneal metastasis of gastric cancer was much higher than liver or lymph metastasis. Peritoneal metastatic and non-metastatic NUGC-4 cells were isolated from the mouse model. Cell proliferation was measured using CCK-8 assays, while migration and invasion were investigated in Transwell assays. Proteins involved in epithelial-mesenchymal transition were detected by Western blotting. Metastatic gastric carcinoma cells were more proliferative and invasive than primary NUGC-4 cells. The supernatants of metastatic gastric carcinoma cells notably altered the morphology of HMrSV5 peritoneal mesothelial cells and promoted their epithelial-mesenchymal transition. Moreover, primary or metastatic gastric cancer cells co-cultured with HMrSV5 cells markedly increased cancer cell proliferation and invasiveness. Moreover, peritoneal metastatic gastric carcinoma cells in the presence of HMrSV5 cells exhibited most malignant behaviors. Thus, peritoneal metastatic gastric carcinoma cells exhibited high capacities for proliferation and invasion, and could be used as a new drug screening tool for the treatment of advanced gastric cancer and peritoneal metastatic gastric cancer.
资助项目	Natural Science Foundation of Zhejiang Province[LY18H290006] ; Zhejiang Provincial Medical and Health Science and Technology Projects[WKJ-ZJ-1728] ; Zhejiang Provincial Medical and Health Science and Technology Projects[2016KYB220] ; Program of Zhejiang Provincial TCM Sci-tech Plan[2016ZZ012] ; Program of Zhejiang Provincial TCM Sci-tech Plan[2018ZY006] ; Program of Zhejiang Provincial TCM Sci-tech Plan[2018ZB044] ; Zhejiang Provincial Science and Technology Projects[2018C37045] ; National Natural Science Foundation of China[81573953] ; National Natural Science Foundation of China[81703753]
WOS关键词	EPITHELIAL-MESENCHYMAL TRANSITION ; MESOTHELIAL CELLS ; CANCER ; INVASION ; GROWTH ; EMT
WOS研究方向	Cell Biology ; Geriatrics & Gerontology
语种	英语
出版者	IMPACT JOURNALS LLC
WOS记录号	WOS:000518397000012
资助机构	Natural Science Foundation of Zhejiang Province ; Zhejiang Provincial Medical and Health Science and Technology Projects ; Program of Zhejiang Provincial TCM Sci-tech Plan ; Zhejiang Provincial Science and Technology Projects ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/103843]
专题	中国科学院合肥物质科学研究院
通讯作者	Cheng, Xiangdong
作者单位	1.Univ Chinese Acad Sci, Inst Canc & Basic Med, Dept Gastr Surg, Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China 2.Univ Chinese Acad Sci, Inst Canc & Basic Med, Dept Ultrasound, Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China 3.Zhejiang Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China 4.Zhejiang Chinese Med Univ, Dept Gastrointestinal Surg, Affiliated Hosp 1, Hangzhou 310000, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Wang, Lijing,Xu, Zhiyuan,Hu, Can,et al. Peritoneal metastatic gastric carcinoma cells exhibit more malignant behavior when co-cultured with HMrSV5 cells[J]. AGING-US,2020,12.
APA	Wang, Lijing.,Xu, Zhiyuan.,Hu, Can.,Chen, Shangqi.,Du, Yian.,...&Cheng, Xiangdong.(2020).Peritoneal metastatic gastric carcinoma cells exhibit more malignant behavior when co-cultured with HMrSV5 cells.AGING-US,12.
MLA	Wang, Lijing,et al."Peritoneal metastatic gastric carcinoma cells exhibit more malignant behavior when co-cultured with HMrSV5 cells".AGING-US 12(2020).