MiR-596 down regulates SOX4 expression and is a potential novel biomarker for gastric cancer

doi:10.21037/tcr.2020.02.02

	MiR-596 down regulates SOX4 expression and is a potential novel biomarker for gastric cancer
	Chen, Yongyi 1,2,3; Gong, Wangang 1,2,3; Dai, Wumin 1,2,3; Pan, Zhiwen 1,2,3; Xu, Xiaohong 1,2,3; Jiang, Huifen 1,2,3
刊名	TRANSLATIONAL CANCER RESEARCH
	2020-02-01
卷号	9
关键词	Bioinformatics analysis gastric cancer (GC) immune infiltration miR-596 SOX4
ISSN号	2218-676X
DOI	10.21037/tcr.2020.02.02
通讯作者	Jiang, Huifen(jianghuifentougao@163.com)
英文摘要	Background: Gastric cancer (GC) is one of the most commonly diagnosed malignancies of the human digestive tract, and currently there is a dearth of effective biomarkers for this disease. Methods: MiR-598 expression levels were analyzed by the cancer genome atlas (TCGA database) mining and verified in GC patient plasma using real-time reverse transcription polymerase chain reaction (RTPCR) assay. We used the GEPIA and UALCAN databases and immunohistochemistry (IHC) to analyze SOX4 expression. The MTT assay assessed MNK28 and SGC7901 cell proliferation after transfection with miR-596 plasmids. The analytical tools, Functional Enrichment Analysis Tool (FunRich), Database of Immune Cell Expression (DICE) and Tumor IMmune Estimation Resource (TIMER) were used to analyze correlations between SOX4 and immune responses. Furthermore, a Kaplan Meier plotter database explored correlations between miR-596, SOX4 and overall patient survival. Results: Data from TCGA and RT-PCR indicated that miR-598 was lowly expressed in GC patients. The miRWalk database showed that SOX4 was the target genes of miR-596 and also revealed that miR-596 bound directly to SOX4. MiR-596 over-expression further depressed GC cell proliferation. In addition, the FunRich database showed that SOX4 was involved in immune responses, and was further shown to be differentially expressed in CD4+ T cells by DICE. Specifically, TIMER indicated that high expression of SOX4 was negatively correlated with infiltrating CD4+ T cells in stomach adenocarcinoma (STAD). Moreover, high expression of miR-596 and low expression of SOX4 prolonged the overall survival (OS) of GC patients. Conclusions: Our study reveals a crucial role for miR-596 in tumor-associated immune infiltration and predicting prognoses in GC patients.
资助项目	Zhejiang Province Public Welfare Technology Application Research Project[2018KY294]
WOS关键词	MICRORNAS ; INVASION
WOS研究方向	Oncology
语种	英语
出版者	AME PUBL CO
WOS记录号	WOS:000518406300094
资助机构	Zhejiang Province Public Welfare Technology Application Research Project
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/103785]
专题	中国科学院合肥物质科学研究院
通讯作者	Jiang, Huifen
作者单位	1.Chinese Acad Sci, Inst Canc Res & Basic Med Sci, Hangzhou 310022, Peoples R China 2.Univ Chinese Acad Sci, Canc Hosp, Dept Clin Lab, Hangzhou 310022, Peoples R China 3.Zhejiang Canc Hosp, Dept Clin Lab, 1 East Banshan Rd, Hangzhou 310022, Peoples R China
推荐引用方式 GB/T 7714	Chen, Yongyi,Gong, Wangang,Dai, Wumin,et al. MiR-596 down regulates SOX4 expression and is a potential novel biomarker for gastric cancer[J]. TRANSLATIONAL CANCER RESEARCH,2020,9.
APA	Chen, Yongyi,Gong, Wangang,Dai, Wumin,Pan, Zhiwen,Xu, Xiaohong,&Jiang, Huifen.(2020).MiR-596 down regulates SOX4 expression and is a potential novel biomarker for gastric cancer.TRANSLATIONAL CANCER RESEARCH,9.
MLA	Chen, Yongyi,et al."MiR-596 down regulates SOX4 expression and is a potential novel biomarker for gastric cancer".TRANSLATIONAL CANCER RESEARCH 9(2020).